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To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
When producing a recombinant plasmid, the plasmid and foreign DNA are cut with the same restriction enzyme(s) to generate complementary sticky ends for ligation. Using different restriction enzymes would create incompatible ends that cannot be ligated together effectively, making it difficult to form a functional recombinant plasmid.
Is it true that If you cut two sources of DNA with the same restriction enzyme you can then hook them together to form recombinant DNA.?
Yes, it is true that if you cut two sources of DNA with the same restriction enzyme, you can create compatible ends that allow them to be joined together to form recombinant DNA. The restriction enzyme creates specific cuts in the DNA, generating sticky or blunt ends that can anneal with each other. Once the DNA fragments are aligned, DNA ligase can be used to seal the nicks in the sugar-phosphate backbone, resulting in a stable recombinant DNA molecule. This technique is widely used in genetic engineering and biotechnology.
Why restriction enzyme is also called endonuclease enzyme?
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
An enzyme called what is added to attach the DNA backbone between vector and foreign DNA that have formed base pairs at the sticky ends during the formation of recombinant dna?
D ligase
What clinical trial offers hope for treatment of Pompe disease?
a recombinant human acid alpha-glucosidase enzyme is being used as a potential enzyme replacement therapy. This recombinant form allows scientist to make a lot of protein
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
When producing a recombinant plasmid, the plasmid and foreign DNA are cut with the same restriction enzyme(s) to generate complementary sticky ends for ligation. Using different restriction enzymes would create incompatible ends that cannot be ligated together effectively, making it difficult to form a functional recombinant plasmid.
Why restriction enzyme is also called endonuclease enzyme?
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
Which enzyme is added to attach the DNA backbone between vetor and foreign DNA that have formed bases pairs at sticky ends during of recombinant DNA?
DNA ligase is added.
Make examples of recombinant protein?
A recombinant protein is a protein that is derived from recombinant DNA.Using recombinant DNA and inserting it to a plasmid of rapidly reproducing bacteria enables the manufacture of recombinant protein. These recombinant proteins can be variety of types, the can be Antibodies, antigens, hormones and enzymes.
An enzyme called what is added to attach the DNA backbone between vector and foreign DNA that have formed base pairs at the sticky ends during the formation of recombinant dna?
D ligase
DNA molecules that are produced by combining DNA from different sources are called?
The word you're looking for may be "recombinant".
What is the substance required to cleave the vector DNA during recombinant DNA technology?
Restriction enzymes are the substances required to cleave the vector DNA during recombinant DNA technology. These enzymes recognize specific DNA sequences and cut the DNA at specific points, allowing for the insertion of foreign DNA fragments.
What enzyme is used to dissolve heart tissue into individual cells?
First a specialized detergent is used without affecting the integrity of the protein in the tissue and then recombinant endonuclease is used to degrade Nucleic acid.
