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Benedict's reagent is used as a test for the presence of reducing sugars.

It is often used in place of Fehling's solution.

This includes all monosaccharides and the disaccharides lactose and maltose.

Even more generally, Benedict's test will detect the presence of aldehydes and alpha-hydroxy-ketones, including those that occur in certain ketoses. Thus, although fructose is not strictly a reducing sugar, it is an alpha-hydroxy-ketone, and gives a positive test because it is converted into the aldoses glucose and/or mannose by the base (carbonate) in the reagent.

One litre of Benedict's reagent can be prepared from 100 g of anhydrous sodium carbonate, 173 g of sodium citrate and 17.3 g of copper(II) sulfate pentahydrate.

Benedict's reagent contains blue copper(II) ions (Cu2+) which are reduced to copper(I) (Cu+). These are precipitated as red copper(I) oxide (Cu2O) which is insoluble in water.

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