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I have never used this before; however, it seems you add 10g of tributyrin per liter of nutrient media for fungi.

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13y ago

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How would expired tributyrin agar affect the result of lipase plus and lipase organisms?

Expired tributyrin agar may lead to decreased pH levels, resulting in a slower breakdown of tributyrin by lipase enzymes and lipase-producing organisms. This could affect the rate of hydrolysis of tributyrin into butyric acid, impacting the ability to accurately assess lipase activity. Additionally, expired agar may also lead to microbial contamination, affecting the growth of specific organisms and potentially skewing the results.


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