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How can you determine retention time of a compound?

Retention time of a compound can be determined using chromatography techniques such as gas chromatography or high-performance liquid chromatography. It is the time taken for a compound to travel through the chromatography system and elute from the column. By comparing the retention time of the compound of interest to known standards, the identification of the compound can be made.


How can one determine the retention time in a chromatography experiment?

Retention time in chromatography can be determined by measuring the time it takes for a compound to travel through the chromatography column and reach the detector. This time is unique to each compound and can be used to identify and quantify substances in the sample.


How do you use chromatography in daily life?

to measure time


What is dead volume HPLC?

The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.


What is the retention time formula used in chromatography to calculate the time a compound spends in the stationary phase?

The retention time formula in chromatography is calculated by dividing the time the compound spends in the stationary phase by the time it takes for the mobile phase to travel through the column.


What are the different types of chromatography?

there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography


What are 3 types of chromatography?

1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography


What is the significance of retention time in chromatography and how does it impact the separation of compounds in a sample?

Retention time in chromatography is the time it takes for a compound to travel through the chromatography column. It is significant because it helps in identifying and separating different compounds in a sample based on their unique retention times. Compounds with different retention times will elute at different times, allowing for their separation and analysis.


How many types of Chromatography are there?

Types of Chromatography:Gas ChromatographyLiquid ChromatographyIon Exchange ChromatographyAffinity Chromatography


How is paper chromatography used in everyday life?

it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.


How do you do chromatography?

I f you want to observe this theory in simple way ,you can observe it through using filter paper has ink on it and leave it in water ,after time when the water get evaporated you will observe that the ink was separated and this is a simple chromatography. And this is core concept for chromatography. Saleh Qahtani Lab Tech


What is chromatography in science?

chromatography seperates substances from an object