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Infections caused by this include urinary tract infections, bacteremia, bacterial endocarditis, diverticulitis, and meningitis. It some times is seen after neurological surgery. It can be treated with ampicillin, penicillin and vancomycin. Some strains are vancomycin resistant and it has become a growing problem in the USA. It s found normally in the lower GI tract, soil, and some waters.
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What is the test that separates enterococcus faecalis and lactococcus lactis?
To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
Does enterococcus faecalis have a positive methyl red test?
Enterococcus faecalis typically does not produce significant amounts of acid from glucose fermentation, so it usually gives a negative result in the methyl red test, which detects mixed acid fermentation.
What is Proteus vulgaris on the Blood Hemolysis Test?
Proteus vulgaris is a bacterium that shows beta hemolysis on blood agar plates. In the Blood Hemolysis Test, this means that Proteus vulgaris will cause complete lysis of red blood cells, resulting in a clear zone around the bacterial growth on the agar plate.
What tests would separate micrococcaceae from the streptococcaceae?
One test to differentiate between Micrococcaceae and Streptococcaceae is the catalase test. Micrococcaceae are catalase-positive, producing bubbles when hydrogen peroxide is added, while Streptococcaceae are catalase-negative, showing no bubbles. Another test is the hemolysis pattern on blood agar plates – Micrococcaceae typically exhibit gamma hemolysis (no hemolysis), while Streptococcaceae may show alpha or beta hemolysis.
What effect does hemolysis have on CO2 values?
Hemolysis can falsely increase CO2 values in blood samples because red blood cells release CO2 when they are broken down. This can interfere with accurate measurement of CO2 levels in the blood. It is important to differentiate between true changes in CO2 levels and those caused by hemolysis when interpreting test results.
What is the test that separates enterococcus faecalis and lactococcus lactis?
To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
Does enterococcus faecalis have a positive methyl red test?
Enterococcus faecalis typically does not produce significant amounts of acid from glucose fermentation, so it usually gives a negative result in the methyl red test, which detects mixed acid fermentation.
What is Proteus vulgaris on the Blood Hemolysis Test?
Proteus vulgaris is a bacterium that shows beta hemolysis on blood agar plates. In the Blood Hemolysis Test, this means that Proteus vulgaris will cause complete lysis of red blood cells, resulting in a clear zone around the bacterial growth on the agar plate.
Can a blue top hemolyze?
Yes, blue top tubes can cause hemolysis if they are shaken too vigorously during blood collection. Hemolysis can release hemoglobin from red blood cells into the serum, affecting test results. It is important to follow proper blood collection techniques to prevent hemolysis.
What tests would separate micrococcaceae from the streptococcaceae?
One test to differentiate between Micrococcaceae and Streptococcaceae is the catalase test. Micrococcaceae are catalase-positive, producing bubbles when hydrogen peroxide is added, while Streptococcaceae are catalase-negative, showing no bubbles. Another test is the hemolysis pattern on blood agar plates – Micrococcaceae typically exhibit gamma hemolysis (no hemolysis), while Streptococcaceae may show alpha or beta hemolysis.
What are the characteristics and implications of partial hemolysis in blood samples?
Partial hemolysis in blood samples refers to the breakdown of some red blood cells, leading to a pink or red discoloration of the sample. This can affect the accuracy of certain blood tests, as it may release substances that interfere with the results. It is important to properly handle and store blood samples to prevent hemolysis and ensure reliable test results.
What effect does hemolysis have on CO2 values?
Hemolysis can falsely increase CO2 values in blood samples because red blood cells release CO2 when they are broken down. This can interfere with accurate measurement of CO2 levels in the blood. It is important to differentiate between true changes in CO2 levels and those caused by hemolysis when interpreting test results.
What is alppha hemolytic?
Alpha hemolysis (α-hemolysis) means that the bacterial enzymes only partially break down the blood cells. This results in the media showing a yellowish/greenish/brownish discoloration (like a bruise) around the colonies, indicating incomplete hemolysis.How to Interpret Beta Hemolysis on Blood Agar: Test for Strep Throat - Identifying B-hemolytic Streptococcus
How does hemolysis affect hematocrit?
Clinically significant hemolysis is rare problem but it can affect red blood cell count and density of plasma. Studies have been done about the effect of hemolysis on hematocrit and the results stated that the changes appear too small to be accurately detected.
What shaking vigorously a blood filled tube will cause?
Shaking a blood-filled tube vigorously can cause hemolysis, which is the rupture of red blood cells leading to the release of hemoglobin into the surrounding fluid. This can affect blood test results by altering the concentrations of certain analytes. It is recommended to gently invert blood tubes to mix contents without causing hemolysis.
What is a Coombs' test?
An indirect Coombs' test looks for antibodies to someone else's red blood cells in the patient's serum
Why is catalase test performed on enterococcus faecium?
The catalase test is performed on Enterococcus faecium to differentiate it from certain other bacteria such as Staphylococcus species, which are catalase-positive. Enterococcus faecium is catalase-negative, meaning it does not produce the enzyme catalase, which helps in the identification of the bacteria.
