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When was gene sequencing discovered?
Gene sequencing was first developed in the late 1970s and early 1980s. The first complete sequencing of a genome, the bacteriophage MS2, was accomplished in 1976, and the first sequencing of a full-length DNA molecule, the bacteriophage φX174, was achieved in 1977.
How is find the gene sequence of an monoclonal antibody found?
find the protein sequence in protein sequence data base.....then from the protein sequence u can find the antibody gene sequence...... u can also go for nucleotide sequencing....which will directly help u in getting the sequence.....u can check this in bioinformatics data base
Technique can be used to determine the expression pattern of Factor VIII gene?
To determine the expression pattern of the Factor VIII gene, techniques such as quantitative PCR (qPCR) or RNA sequencing (RNA-seq) can be employed. These methods allow for the quantification of mRNA levels, revealing how and when the Factor VIII gene is expressed in different tissues or under various conditions. Additionally, in situ hybridization can visualize the spatial expression of the gene within tissues. Collectively, these techniques provide insights into the regulation and functional significance of Factor VIII in hemostasis.
What does ngs go mean?
"NGS" most likely refers to Next-Generation Sequencing, a high-throughput technique used to sequence DNA or RNA. "GO" could stand for Gene Ontology, a system for classifying genes and their functions. Together, "NGS GO" might refer to the analysis of gene expression data generated through next-generation sequencing using gene ontology terms.
What types of DNA sequencing in their DNA?
The types of DNA sequencing are whole-genome sequencing which maps entire DNA sequences, targeted sequencing which focuses on specific genomic regions, and RNA sequencing which identifies gene expression levels.
How can one determine the gene sequence of a specific organism?
To determine the gene sequence of a specific organism, scientists use a process called DNA sequencing. This involves isolating the DNA from the organism, breaking it into smaller fragments, sequencing these fragments, and then assembling the sequences to determine the complete gene sequence. Various technologies and methods, such as next-generation sequencing, are used to accurately determine the order of nucleotides in the DNA.
What does DNA isolation used for?
Gene sequencing and gene cloning
How does RNA sequencing work and what are the key steps involved in the process?
RNA sequencing is a technique used to analyze the expression of genes in a cell. The key steps involved in the process include isolating RNA from the cell, converting it to complementary DNA (cDNA), sequencing the cDNA fragments, and analyzing the data to determine gene expression levels.
What is DNA sequencing used for?
determine the rick of developing certain diseases
When was gene sequencing discovered?
Gene sequencing was first developed in the late 1970s and early 1980s. The first complete sequencing of a genome, the bacteriophage MS2, was accomplished in 1976, and the first sequencing of a full-length DNA molecule, the bacteriophage φX174, was achieved in 1977.
How would you determine the size of a gene?
To determine the size of a gene, scientists typically use techniques such as DNA sequencing or polymerase chain reaction (PCR) to analyze the specific sequence of nucleotides that make up the gene. By comparing the sequence to known genetic information, researchers can estimate the size of the gene based on the number of nucleotides it contains.
What does Sanger sequencing do and how is it used in genetic analysis?
Sanger sequencing is a method used to determine the order of nucleotides in a DNA molecule. It is commonly used in genetic analysis to identify genetic variations, mutations, and sequences of genes.
What is the meant by the term rapid sequencing?
Rapid Sequencing. Faster way of DNA sequencing using Computers and cutting down the timeline of the Human genome project. Gene Therapy. The insertion of working copies of a gene into the cells of a person with a genetic disorder in an attempt to correct the disorder.
Why would a person use Sanger sequencing?
Sangers sequencing technique is used to determine the order of nucleotides in a strand of DNA (deoxyribonucleic acid). It was first used to discover the order of the nucleotides in the genomes of various organisms during the Human Genome Project.
What methods are used to determine phylogenies?
Phylogeny is discovered using molecular sequencing data and morphological data matrices.
What are gene map's and how are they produced?
Gene maps show the location of genes on a chromosome. They are produced using data from genetic markers that are known to be located near specific genes. Techniques like genetic linkage analysis and genome sequencing are used to determine the order and distance between genes on a chromosome.
How is find the gene sequence of an monoclonal antibody found?
find the protein sequence in protein sequence data base.....then from the protein sequence u can find the antibody gene sequence...... u can also go for nucleotide sequencing....which will directly help u in getting the sequence.....u can check this in bioinformatics data base
