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A technique where a long chain of same (homo) nucleotides (polymer) are attached to the 3' OH end. This procedure is very useful for subcloning :
(Suppose the one in bold is the target gene and the other is your vector)
5'------------- AA(An)AA-OH --------------3'
+
3'------------- OH-TT(Tn)TT--------------5'
|
|DNA Ligase permanently joins the fragments together
|
V
5'------------- AA(An)AA------------- 3'
3'--------------TT(Tn)TT--------------5'
The bad part is you can't control the length.
Look up Terminal transferases for more details.
What else can I help you with?
Why polystyrene is homopolymer?
Polystyrene is formed from only one monomer - styrene.
What is a homo polymer?
a polymer which is obtained by the polmerisation of only one type of monomer is known as a homopolymer eg:teflon, polyetheleyne etc...if it is constituted of more than one type of monomer( simpler units) then the polymer is known as copolymereg:dacron, buna s etc
What are the causes of tailing in chromatography?
Tailing in chromatography can be caused by factors such as non-specific interactions between the analyte and the stationary phase, column overload, sample matrix effects, or inaccuracies in the injection process. These factors can lead to peak distortion, decreased resolution, and decreased sensitivity in chromatographic separations.
Is Myosin a homopolymer or a heteropolymer?
Myosin is a heteropolymer because it consists of multiple subunits with different amino acid sequences that come together to form the final protein structure. The assembly of these subunits results in the functional myosin protein.
What is Something that is made up of repeating monomer units?
A polymer is a substance composed of repeating monomer units, where the monomers are linked together through chemical bonds to form a larger molecule. Examples of polymers include plastics, proteins, and DNA.
Polyphenyl is homopolymer or copolymer?
Homo
Explain the trends in e-tailing in India?
explain in the trends in e-tailing in India
Why polystyrene is homopolymer?
Polystyrene is formed from only one monomer - styrene.
Do tailing ponds evaborate?
That's a terrible question. Of course tailing ponds evaporate. Use your head before asking questions.
What actors and actresses appeared in Tailing the Millennium - 2000?
The cast of Tailing the Millennium - 2000 includes: Anna Zetchus as Herself - Narrator
How did the miners dispose waste?
A ditch is made beside a tree and the tailing is dumped into it, and then, the tailing is covered with earth. This method is known as Phytostabilisation.
What is fish-tailing for vehicle driving safety?
Fish-tailing is were the back of the car loses traction and starts to slide side to side. If not corrected the car will spinout.
What is tailing effect in TLC?
Tailing effect is an error that occurs when the spot is not allowed to dry hence leading increased diameter of the spot in a TLC plate. Nwando Ata.
Which type of plastic are plastic shopping bags made of?
They can be made from several different things: Butene Copolymer Copolymer - HDPE (High Density Polyethylene) Ethylene Vinyl Acetate HDPE - Reclaim LLDPE (Linear Low Density Polyethylene) -Hexene Copolymer Homopolymer - HDPE Homopolymer - LDPE (Low Density Polyethylene) Homopolymer - PP (Polypropylene) PP-IM (Impact) LDPE-Reclaim LLDPE-OC (Octene Copolymer) LDPE Copolymer For more information go to http://www.townsendpolymer.com
How are guilt by association and coat tailing related?
beacuae its just is
What is fish tailing?
A fish's tail is called a caudal fin.
How do you properly dispose of mining waste?
The mining waste is ,usually, called 'tailing'. We dispose of tailings in many ways like:-1. We build a pond and the tailing is pumped into it to allow sedimentation. This is beneficial as it forbids the fine particles to mingle with air causing air pollution.2. Nowadays, these are also dumped as landfills in required ares.3. If there is a hole or a trench that is to be filled, then also this tailing is used. In this case, it is mixed with cement and then is used as a filling material.4. Recently, a ditch is made beside a tree and the tailing is dumped into it, and then, the tailing is covered with earth. This method is known as Phytostabilisation.
