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How do you optimise peak separation in HPLC?

Peak separation in HPLC can be optimized by adjusting parameters such as column length, particle size, mobile phase composition, flow rate, and temperature. Increasing column length can improve separation, while decreasing particle size can enhance resolution. Adjusting mobile phase composition can also impact peak separation by changing selectivity. Optimization is typically achieved through systematic experimentation and adjustment of these parameters.


What pore size is related with porosity 2 of sintered glass filter?

The pore size is the average 100 micrometers.


What is the pore size of seitz filter?

Seitz filters typically have a pore size ranging from 0.1 to 1 micron. The specific pore size will depend on the manufacturer and the intended application of the filter. It's important to select the appropriate pore size based on the particles you need to remove from the liquid.


How do you select hplc column in method development?

firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column. after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud


What has larger pores 25 or 50 micron?

If the 25 and 50um refer to the actual pore size, then the 50 would be larger pore size


What pore size does porosity 4 sintered glass have?

Porosity 4 sintered glass typically has pore sizes ranging from 4 to 16 micrometers, with an average pore size of around 8 micrometers. The pore size distribution can vary depending on the specific manufacturing process and conditions used to produce the sintered glass material.


What is the Pore size in hepa filter?

0.2 micron


What the fbd fingerbag pore size?

250 micron


What is porosity and pore size in glass?

No, glass is not porous


What does pore size mean?

Pore size refers to the diameter of the openings in materials like membranes or filters. It is a measurement of how small or large the spaces are through which substances can pass. Smaller pore sizes can filter out smaller particles, while larger pore sizes allow larger particles to pass through.


How is protein purification achieved using HPLC techniques?

Protein purification using HPLC techniques involves separating proteins based on their size, charge, and hydrophobicity. The process involves passing a protein mixture through a column filled with a stationary phase that interacts with the proteins differently, allowing for their separation. The proteins are then eluted from the column using a solvent gradient, with each protein eluting at a different time based on its unique characteristics. This results in the isolation of pure proteins for further analysis or use.


What determines the pore size of a polyacrylamide gel?

The pore size of a polyacrylamide gel is primarily determined by the concentration of acrylamide and the crosslinker used in the gel preparation. Higher concentrations of both acrylamide and crosslinker result in smaller pore sizes, while lower concentrations lead to larger pore sizes. Additionally, the ratio of acrylamide to crosslinker can also impact pore size.