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Mixed retention mechanism in liquid chromatography refers to a separation process that combines both polar and non-polar interactions between the stationary phase and the analytes. This approach utilizes both hydrophobic (reversed-phase) and polar (normal-phase) interactions to enhance the selectivity and resolution of the separation. By employing a stationary phase that incorporates both types of interactions, mixed retention mechanisms can effectively separate compounds with varying polarities, allowing for more versatile applications in complex mixture analyses.

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1mo ago

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