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a protocol for plasmid curing in enteric bacteria using acridine orange

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What is curing of plasmid?

Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes). Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange!


Why does acridine orange fluoresce in green light only?

Acridine orange fluoresces in green light because it absorbs blue light and emits green light due to its specific molecular structure and energy levels. This phenomenon is known as fluorescence resonance energy transfer (FRET) which allows acridine orange to emit green light when excited by blue light.


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