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The most important component depends on where you are looking at it. The most important organelle in forming ATP is the mitochondria which undergo cellular respiration and oxidative phosphorylation to produce tons of ATP in eukaryotic cells. Bacteria (prokaryotes) do not need this organelle because their respiration and phosphorylation happen in the cell cytoplasm. The most important enzyme in the synthesis of ATP is the ATPase, which is dependent on the protomotor force (movement of hydrogen cations also known as protons) generated by the cell. When enough protons move across to the extracellular space, they come back into the cytoplasm, then ADP and an inorganic phosphate (Pi) bind to ATPase to make ATP. This enzyme is in both eukaryotes and prokaryotes.

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