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Configuration of the GFP inside bacterial cells?
Inside bacterial cells, the green fluorescent protein (GFP) is typically encoded by a gene that can be introduced into the bacterial genome or expressed on a plasmid. The gene consists of coding sequences that allow the production of the GFP protein, which fluoresces green when exposed to specific wavelengths of light. The gene is regulated by bacterial promoters and terminators to control its expression level. The GFP protein is then synthesized within the bacterial cell and can be visualized using fluorescence microscopy or other techniques.
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
What would have happened if we had cut both the jellyfish glo gene and puc18 plasmid with the ecoR1 restriction enzyme?
If both the jellyfish GFP gene and the puc18 plasmid were cut with the EcoRI restriction enzyme, they would produce complementary sticky ends that can anneal together. This allows for the insertion of the GFP gene into the puc18 plasmid, facilitating the creation of a recombinant DNA molecule. This process is essential for cloning the GFP gene, which can then be used for various applications, such as gene expression studies or protein production in host cells. Additionally, transformation of the modified plasmid into a suitable host would enable the expression of the GFP protein.
Which organelles inside bacteria make green fluorescent protein?
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
Which parts of a gene are expressed as protein?
Coding sequences of a gene are expressed as protein
Configuration of the GFP inside bacterial cells?
Inside bacterial cells, the green fluorescent protein (GFP) is typically encoded by a gene that can be introduced into the bacterial genome or expressed on a plasmid. The gene consists of coding sequences that allow the production of the GFP protein, which fluoresces green when exposed to specific wavelengths of light. The gene is regulated by bacterial promoters and terminators to control its expression level. The GFP protein is then synthesized within the bacterial cell and can be visualized using fluorescence microscopy or other techniques.
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
What would have happened if we had cut both the jellyfish glo gene and puc18 plasmid with the ecoR1 restriction enzyme?
If both the jellyfish GFP gene and the puc18 plasmid were cut with the EcoRI restriction enzyme, they would produce complementary sticky ends that can anneal together. This allows for the insertion of the GFP gene into the puc18 plasmid, facilitating the creation of a recombinant DNA molecule. This process is essential for cloning the GFP gene, which can then be used for various applications, such as gene expression studies or protein production in host cells. Additionally, transformation of the modified plasmid into a suitable host would enable the expression of the GFP protein.
Which organelles inside bacteria make green fluorescent protein?
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
Why use arabinose in the plate in pglo experiments?
Arabinose is used in the plate in pglo experiments to induce the expression of the Green Fluorescent Protein (GFP) gene. The presence of arabinose activates the araC promoter, allowing for the transcription and translation of the GFP gene, which results in the production of green fluorescent protein in the bacteria. This fluorescence helps researchers visualize and track the transformation of the bacteria with the desired gene.
Is an expressed gene turned off?
No. If a gene is expressed, it is turned on.
An expressed gene is one that what?
a gene being expressed means it is shown, for example if you have the gene for brown eyes and the gene for blue eyes, only one can be expressed. if you have brown eyes that gene is expressed, if not the blue-eye gene is expressed.
Which parts of a gene are expressed as protein?
Coding sequences of a gene are expressed as protein
How is a protein tagged to a fluorescent protein?
The most common protein that is used as a 'tag' for other proteins is GFP. In order to do this, the GFP gene sequence is simply added at the end of the gene sequence for the target protein. GFP is particularly useful for this because it contains its own chromophore, and needs nothing else except the gene sequence to create its own light. This makes possible easy tracking of certain proteins without necessarily killing the tester animal.
What does it mean for a gene to be off?
How is a gene not expressed
What is glo gene?
Usually used in GMOs (genetically modified organism) as 'markers' when a plant is genetically engineered to see if it has taken up the new gene. GLO-gene becomes visible in the organism when put under ultraviolet light.
How can genetic engineering be used to make a transgenic tobacco plant that glows in the dark?
Agrobacterium-mediated transformation using a plasmid containing a gene for GFP
