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peptone iron agar is used to demonstrate the production of hydrogen sulfide. ıt is more sensitive than lead acetate agar. some organisms have the enzyme to liberate hydrogen sulfide from sulfur containing organic compounds-such as protein-. when H2S is liberated by the enzyme,, the sulfur of the H2S combines with the iron in the PIA medium to form an iron sulfide. The iron sulfide is manifested as a black precipitate in the medium. so you can use in the separation and identification of bacteria. Peptone Iron Agar Approximate Formula* Per Liter Peptone ................................................................... 15.0 g Proteose Peptone ....................................................... 5.0 g Ferric Ammonium Citrate ........................................... 0.5 g Sodium Glycerophosphate ......................................... 1.0 g Sodium Thiosulfate .................................................... 0.08 g Agar ......................................................................... 15.0 g

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