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Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
What else can I help you with?
What is the working volume in a 500ml shake flask?
The working volume in a 500ml shake flask is typically around 20-25% less than the total flask volume, so it would be around 375-400ml. This allows for proper aeration and mixing of the culture without overflowing during shaking.
What is the principle of shake-flask extraction?
Shake-flask extraction is a liquid-liquid extraction technique used to separate compounds based on their solubility in two immiscible liquids, typically water and an organic solvent. In this process, a mixture containing the target compound is placed in a flask along with the organic solvent, and the flask is shaken to promote mixing. The target compounds preferentially dissolve in one of the phases, allowing for their separation from the other components in the mixture. After shaking, the two phases separate, and the desired compound can be collected from the phase in which it is more soluble.
What is flat bottom flask?
A flat bottom flask would be an Ehrlemeyer flask, which is used quite a lot by chemists.anf
A flask fitted with a stopper that is used in the preparation of solution?
volumetric flask
How do clean a beaker?
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.
What are design aspects of shake flask fermentation?
Design aspects of shake flask fermentation include selecting appropriate flask volume and shape, ensuring proper sealing to prevent contamination, choosing an appropriate stirring mechanism and speed, controlling temperature and aeration levels, and monitoring and adjusting pH and nutrient levels as needed. These aspects are crucial for optimizing microbial growth and product yield in shake flask experiments.
What is the working volume in a 500ml shake flask?
The working volume in a 500ml shake flask is typically around 20-25% less than the total flask volume, so it would be around 375-400ml. This allows for proper aeration and mixing of the culture without overflowing during shaking.
What is the principle of shake-flask extraction?
Shake-flask extraction is a liquid-liquid extraction technique used to separate compounds based on their solubility in two immiscible liquids, typically water and an organic solvent. In this process, a mixture containing the target compound is placed in a flask along with the organic solvent, and the flask is shaken to promote mixing. The target compounds preferentially dissolve in one of the phases, allowing for their separation from the other components in the mixture. After shaking, the two phases separate, and the desired compound can be collected from the phase in which it is more soluble.
What is another word for triangular flask?
Another word for a triangular flask is a conical flask.
What is a small alcohol container called?
UMMM....A bottle? i think it's called a decanter
What is a filter flask used for?
what is a flask for what is a flask for
How to clean a flask effectively?
To clean a flask effectively, first rinse it with warm water. Then, fill it with a mixture of warm water and dish soap, and shake it well. Use a bottle brush to scrub the inside, and rinse thoroughly. For tough stains, use a mixture of baking soda and water or white vinegar. Let it sit for a few hours before rinsing again. Finally, air dry the flask upside down.
Why the titration flask need to be shake during titration?
Shaking the titration flask during titration helps to ensure that the reaction mixture is well-mixed and that the titrant is evenly distributed throughout the solution. This promotes a more uniform reaction and more accurate measurement of the endpoint of the titration.
What is another word for flask?
erlenmeyer flask
What is flat bottom flask?
A flat bottom flask would be an Ehrlemeyer flask, which is used quite a lot by chemists.anf
What is elengi flask?
Nice flask Elengi means nice, beautiful, brilliant etc........in short its nice flask
What are some unique gift ideas for a friend who loves collecting flask-shaped items?
Some unique gift ideas for a friend who loves collecting flask-shaped items could include a custom-engraved flask, a vintage or antique flask, a flask-shaped keychain, a flask-shaped phone case, or a flask-shaped decorative item for their home.
