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A single beam spectrophotometer is a type of spectrophotometer that measures the intensity of light at a specific wavelength. It uses a single beam of light that passes through a sample and a reference solution to determine the absorbance. Single beam spectrophotometers are simpler in design and are typically more affordable than double beam spectrophotometers.

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What is the purpose of collimating slits in a spectrophotometer and how do they affect the accuracy of the measurements?

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How Does a Double Beam Spectrophotometer Improve Measurement Accuracy?

Simultaneous Reference and Sample Measurement: By comparing the sample to a reference beam in real-time, it compensates for fluctuations in light intensity, ensuring more reliable readings. Reduced Error: The use of dual beams minimizes errors caused by external factors like lamp instability or changes in environmental conditions. Ideal for Complex Samples: This instrument is especially effective when measuring complex or highly absorbent samples, providing greater precision compared to single-beam models.


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