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How the hydrogen bonds break when the restriction endonuclease enzyme acts on the sugar - phosphate backbone of a DNA?

Restriction endonucleases break hydrogen bonds between complementary base pairs in DNA, not the hydrogen bonds in the sugar-phosphate backbone. These enzymes recognize and bind to specific DNA sequences, then cleave the phosphodiester bonds in the backbone at specific locations, resulting in DNA fragmentation.


What does not read DNA sequences?

People not versed in DNA sequencing.


What do palindrome have to do with the way restriction enzymes cut DNA?

Palindrome sequences in DNA are important for the way restriction enzymes cut DNA because these enzymes recognize specific palindrome sequences and cut the DNA at specific points within these sequences. Palindrome sequences are symmetrical sequences of nucleotides that read the same forwards and backwards, allowing restriction enzymes to identify and bind to these sequences for cleavage. This specificity is crucial for the precise cutting of DNA at desired locations.


What similarities in DNA and protein sequences do humans and chimpanzees share?

Humans and chimpanzees share about 98.7 of their DNA sequences and have similar protein sequences due to their close evolutionary relationship.


What is the name of the substance that is used to cut DNA at particular sequences?

DNA is cut by a special kind of enzymes called restriction enzymes.


What percent of human DNA sequences are identical across individuals?

Approximately 99.9 of human DNA sequences are identical across individuals.


What if we could see the individual nucleotide sequences of DNA?

You can see the nucleotide sequences in the DNA. It is called as DNA finger printing. It has got many applications in molecular biology.


What can cause changes in DNA sequences?

mutations


Why are DNA sequences more similar in more closely related organisms?

DNA sequences are more similar in closely related organisms because they share a common ancestor and have undergone fewer genetic changes over time. As organisms diverge and evolve, mutations accumulate in their DNA, leading to differences in their genetic sequences. Therefore, closely related organisms have had less time to accumulate mutations, resulting in more similar DNA sequences.


How do restriction enzymes work to cut DNA molecules at specific sequences?

Restriction enzymes work by recognizing specific sequences of DNA called recognition sites and cutting the DNA at those sites. These enzymes are like molecular scissors that can identify and bind to particular sequences of DNA, then cut the DNA at specific points within those sequences. This process allows scientists to precisely manipulate and study DNA molecules.


What are palindromes in biology?

In biology, palindromes refer to specific DNA sequences that read the same forwards and backwards. These sequences are important for DNA replication and repair processes. Palindromic sequences are also commonly found in restriction enzyme recognition sites.


What are the specific sequences found at the 3' and 5' ends of DNA molecules?

The specific sequences found at the 3' and 5' ends of DNA molecules are known as the 3' end and 5' end, respectively. These sequences are important for DNA replication and transcription processes.