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The blue band at the bottom of the gel is Bromophenol blue and DNA fragments will be in the gel according to their molecular weight.

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Are the DNA fragments in band 4 larger or smaller than those of band 1?

The size of DNA fragments in band 4 should be smaller than those of band 1. The fragments can be separated by electrophoresis, with the smaller fragments migrating farther than the larger ones.


Why is the largest DNA fragment band found closest to the well in which it was placed?

The largest DNA fragments travel more slowly through the agarose gel due to their size, so they don't move as far from the well as smaller fragments during gel electrophoresis. This results in the largest fragments being closest to the well after electrophoresis is completed.


Does a single band form in electrophoresis gel if all the DNA fragment is of same size?

if all the fragments are of same size (same fragment length), it will form a single band on an agarose gel. unless you are using other gel electrophoresis technique like DGGE, then only it will separate fragments with different sequences, but that will depends on the resolution power too.


Suppose you had 500 pieces of each of the four fragments how would the gel appear?

It depends on the length of your four fragments. If the 4 fragments are same in length, you can not distinguis the band on the gel as all the (4x500) molecules run at the same length. If they are different in size let say 100,200,300,400 you can see four distinct bands. The intensity of the bands may be stronger at 400 and drops down to the lower fragments because of the higher molecular mass.


Who sings the first part of lightning?

The song "Lightning" is performed by the boy band "The Wanted". The first line of the song, "You're in control, pressing pause on my heartbeat," is performed by band member Tom Parker.

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Are the DNA fragments in band 4 larger or smaller than those of band 1?

The size of DNA fragments in band 4 should be smaller than those of band 1. The fragments can be separated by electrophoresis, with the smaller fragments migrating farther than the larger ones.


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Why is the largest DNA fragment band found closest to the well in which it was placed?

The largest DNA fragments travel more slowly through the agarose gel due to their size, so they don't move as far from the well as smaller fragments during gel electrophoresis. This results in the largest fragments being closest to the well after electrophoresis is completed.


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Their Facebook page has a pic of a band member giving the finger, I would say no.