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Chemistry of the CHOD-PAP method of cholesterol measurement
The cholesterol is determined after enzymatic hydrolysis and oxidation.
The indicator quinoneimine is formed from hydrogen peroxide and
4-aminoantipyrine in the presence of phenol and peroxidase.
Cholesterol
Cholesterol ester + H2O ----------------------> Cholesterol + Fatty acids
esterase
Cholesterol
Cholesterol + O2 ----------------------------->Cholestene-3-one + H2O2
oxidase
Peroxidase
2H2O2 + phenol + 4-Aminoantipyrine ---------------------------> quinoneimine + 4H2O
What else can I help you with?
What is the direct measurement method of measuring volume?
The direct measurement method involves physically measuring the volume of an object by using instruments such as a ruler, measuring tape, or graduated cylinder. This method provides an accurate measurement of the volume of regular-shaped objects by directly calculating their dimensions.
A chemical or tool that indicates a substance is present is called an?
This is a method of analytical chemistry.
Why did Artturi Ilmari Virtanen win The Nobel Prize in Chemistry in 1945?
The Nobel Prize in Chemistry 1945 was awarded to Artturi Virtanen for his research and inventions in agricultural and nutrition chemistry, especially for his fodder preservation method.
What is the advantage of wet method over dry method in chemistry analyzer?
The advantage of the wet method over the dry method in a chemistry analyzer is that it provides more accurate results due to the use of liquid reagents. The wet method allows for better control of reaction conditions and more precise measurements, making it ideal for analytes that are difficult to detect using dry reagents. Additionally, the wet method offers a wider range of test options compared to the dry method.
Why you use SI method in clinical chemistry?
The SI method, or the International System of Units, is used in clinical chemistry for standardization and consistency in measuring and reporting chemical concentrations. It allows for easy comparison of results across different laboratories and ensures accurate interpretation of patient results. Additionally, the SI method is widely recognized and accepted globally, making it the preferred method for clinical chemistry testing.
What is meant by analysis?
Volumetric analysis (volumetry) is a largely used method in analytical chemistry based on the measurement of a solution volume.
What is the method of making a rough measurement?
A method of making a rough measurement is: Estimation Answered by :PopTy13
Does calamari have a high cholesterol?
Yes. Regardless of cooking method, calamari itself has higher levels of LDL (bad) cholesterol.
What are the advantages of standard method of measurement?
.
How does nuclear chemistry apply to food chemistry?
One method of preservating food is by irradiation.
What is end point method?
The endpoint method, often used in various fields such as chemistry and finance, refers to a technique for determining the conclusion of a process. In titration, for example, it involves identifying the point at which a reaction is complete, typically indicated by a color change or a stable measurement. In finance, it can refer to the point at which an investment reaches its desired outcome or maturity. Overall, the endpoint method is critical for accurately assessing the completion of a given process or measurement.
What method of measurement if volume of liquid?
Liters.
How close or reproducible measurement are?
Reproducibility is one component of the precision of a measurement or test method.
A good method for separating dust from water?
chemistry
What is the direct measurement method of measuring volume?
The direct measurement method involves physically measuring the volume of an object by using instruments such as a ruler, measuring tape, or graduated cylinder. This method provides an accurate measurement of the volume of regular-shaped objects by directly calculating their dimensions.
When is the indirect method appropriate and what are the benefits of using it?
Indirect measurement is a technique that uses proportions to find a measurement when direct measurement is not possible.
What is standard method of measurement 7?
The Standard Method of Measurement known is the SMM7 publish by the the Royal Institution of the Chartered Surveyors is the appropriate method of measurement for building works. The SMM7 rules are simplified enough to produce shorter bill of quantities and the contents updated to conform to modern practice.
