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Composition
* Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C
and protect from direct Sunlight.
Instructions for Use
Dissolve 45gms in 445 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the
medium completely. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. Cool to 45-50°C. Aseptically
add equal amount (445ml) of sterile 2% Haemoglobin powder (TS 021) and add 10ml of Vitamins growth
supplement (TS 022) or Yeast autolysate supplement (TS 023). Mix well. Dispense into sterile Petri plates
or sterile culture tubes.
Appearance: Light amber color, clear to slightly opalescent gel. On addition of Haemoglobin: Chocolate
brown colour appears, opaque gel
pH (at 25°C): 7.3 ± 0.2
Principle
CHOCHOLATE AGAR BASE is used for isolation and cultivation of fastidious microorganisms like
Neisseria gonorrhoeae. It can also be used for cultivation of aerobic, anaerobic and microaerophillic
microorganisms. Medium contains Proteose peptone is a nitrogen source required for the growth of wide
variety of organisms. Dextrose acts a carbon energy source. Disodium phosphate buffers the medium
whereas sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent. This medium
is supplemented with cofactor, which provides NAD to facilitate the growth
of Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis. Heated sheep blood is added to
give the medium its "chocolate" appearance. This medium is prepared, stored and dispensed under
oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation
Cultural characteristics observed after inoculating (103CFU/ml), on incubation at 35 ± 2°C for 48 hours
with CO2.
Ingredients Gms/Ltr.
Proteose peptone 20.0
Agar 15.0
Sodium chloride 5.00
Disodium phosphate 5.00
Dextrose 0.50
Regards
NITIN MALHOTRA
What else can I help you with?
What is composition of starch agar plate?
The composition of staph 110 agar is: Yeast extract - 2.5 g/L Tryptone - 10.0 g/L Lactose - 2.0 g/L Mannitol - 10.0 g/L Sodium Chloride - 75.0 g/L Dipotassium hydrogen phosphate - 5.0 g/L Gelatin - 30.0 g/L Agar - 15.0 g/L
When is a starch agar plate used?
A starch agar plate is used to detect the presence of starch-degrading enzymes, such as amylase. Organisms that produce amylase will break down starch in the agar, creating a clear zone around the growth. This test is often used in microbiology to differentiate between different bacterial species based on their ability to degrade starch.
What is the composition of luria agar?
The composition of luria agar is NaCl,Trypotone, Yeast extract and agar.
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
Why actinomycetes grow on casein starch agar?
Actinomycetes can grow on casein starch agar because it provides a nutrient-rich environment suitable for their growth. Casein provides amino acids for protein synthesis, while starch can be broken down into sugars for energy production through metabolism. The combination of these components in the agar supports the growth and development of actinomycetes.
What is composition of starch agar plate?
The composition of staph 110 agar is: Yeast extract - 2.5 g/L Tryptone - 10.0 g/L Lactose - 2.0 g/L Mannitol - 10.0 g/L Sodium Chloride - 75.0 g/L Dipotassium hydrogen phosphate - 5.0 g/L Gelatin - 30.0 g/L Agar - 15.0 g/L
Is E. coli positive or negative for starch agar test?
E.coli does not digest the starch on a starch agar plate, therefore it does not produce amylase making it negative.
What are the contents of starch agar?
:D Beef extract (3.0g) Soluble Starch (10.0) Agar (12.0) Distilled water (1,000.0 ml)
When is a starch agar plate used?
A starch agar plate is used to detect the presence of starch-degrading enzymes, such as amylase. Organisms that produce amylase will break down starch in the agar, creating a clear zone around the growth. This test is often used in microbiology to differentiate between different bacterial species based on their ability to degrade starch.
Why do you add soluble starch to nutrient agar rather than just growing cultures on potatoes?
Soluble starch is added to nutrient agar to provide a consistent and easily controlled source of carbohydrates for microbial growth, allowing for standardized experiments. Unlike potatoes, which can vary in nutrient composition and may contain inhibitory compounds, nutrient agar with soluble starch ensures uniformity and reproducibility. This also allows for the cultivation of a wider range of microorganisms in a more sterile environment.
Why must use starch casein nitrate agar and what is the starch casein agar properties?
Starch casein nitrate agar is used to culture aerobic spore-forming bacteria, particularly Bacillus species. It promotes sporulation due to the presence of nitrates and starch as a carbon source. The properties of starch casein agar include supporting the growth of fastidious bacteria, promoting sporulation, and enabling the detection of extracellular enzyme production.
Why do you test for starch?
You can test for starch in food by dropping iodine on food on an agar plate, if it turns black, it contains starch.
Why do bacteria grow heavily on starch agar but don't produce alpha amylase?
Starch agar contains beef extract, soluble starch, distilled water, and agar. Beef agar allows a variety of microbes to grow on this agar. Only a handful of those numerous microbes can produce amylase hense they will grow but won't break down starch.
What is the composition of luria agar?
The composition of luria agar is NaCl,Trypotone, Yeast extract and agar.
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
Why actinomycetes grow on casein starch agar?
Actinomycetes can grow on casein starch agar because it provides a nutrient-rich environment suitable for their growth. Casein provides amino acids for protein synthesis, while starch can be broken down into sugars for energy production through metabolism. The combination of these components in the agar supports the growth and development of actinomycetes.
What regent would you add to starch agar plate to detect starch hydrolsis?
Iodine should be sufficent. When starch and iodine are present they react with each other and the starch turns blackish. If your bacteria hydrolise your starch then the area will be clear instead of blackish.
