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What might the consequence be of pouring the plates 2mm deep instead of 4mm?
Pouring plates at 2mm depth instead of 4mm could result in a reduced volume of solid agar available for bacterial growth, potentially leading to decreased colony size and difficulty in isolating or visualizing colonies. Additionally, lower volume may lead to faster agar evaporation, potentially drying out the plates more quickly, affecting the overall quality of the experiment.
What are some factors that might aid or slow diffusion?
Factors that can aid diffusion include high temperature, smaller molecular size, steep concentration gradients, and a larger surface area for exchange. Factors that can slow diffusion include low temperature, larger molecular size, long diffusion distances, and obstacles in the medium through which diffusion is occurring.
How is diffusion determined?
Diffusion is determined by the concentration gradient, temperature, and the size of the molecules. The greater the difference in concentration, the higher the temperature, and the smaller the molecules, the faster diffusion will occur.
What is the size of a diffusion particle?
Diffusion particles can vary in size, ranging from nanoparticles (1-100 nm) to larger molecules or particles. The size of a diffusion particle affects its movement and speed in a medium. Smaller particles generally diffuse more rapidly than larger ones due to their higher mobility.
What factors affect the zone of inhibition?
Pathogen susceptibility influences the zone of inhibition because organisms will not grow if they are susceptible to antibiotics. Another influence are the pH levels of agar which should fall between 7.2 and 7.4 room temperature.
In the diffusion of liquids through agar what characteristic of liquid determines the rate at which it will travel through the agar?
The size of the molecules.
What is the principle of agar diffusion method?
The principle of agar diffusion method is based on the diffusion of an antimicrobial agent from a sample into an agar medium containing a test organism. The size of the zone of inhibition that forms around the sample is used to determine the effectiveness of the antimicrobial agent against the test organism.
Does diffusion effect cell size?
Yes
How does the thickness of agar affect the diameter of the zone of inhibition?
A thicker layer of agar may lead to a larger zone of inhibition due to the increased diffusion distance for the antimicrobial agent. The agar thickness can influence the rate of diffusion of the antimicrobial agent into the agar, impacting the size of the zone of inhibition formed around the antimicrobial disk.
What might the consequence be of pouring the plates 2mm deep instead of 4mm?
Pouring plates at 2mm depth instead of 4mm could result in a reduced volume of solid agar available for bacterial growth, potentially leading to decreased colony size and difficulty in isolating or visualizing colonies. Additionally, lower volume may lead to faster agar evaporation, potentially drying out the plates more quickly, affecting the overall quality of the experiment.
How diffusion and osmosis limit the size of a cell?
becauuse it does
How does osmosis and diffusion limit the size of a cell?
diffusion and osmosis limit the size of a cell by how fast is can move
How does size play a role in diffusion?
no answer.
What factors must be carefully controlled in the kirby-Bauer method?
purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.
What factors must be carefully control in the Kirby-Bauer method?
purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.
What influences the rate of diffusion?
The rate of diffusion is influenced by the concentration gradient, temperature, molecular size, and the medium through which the particles are diffusing. A steeper concentration gradient, higher temperature, smaller molecular size, and a less dense medium all tend to increase the rate of diffusion.
What variables are held constant in osmosis and diffusion?
In osmosis, the concentration gradient, temperature, pressure, and size of the particles are typically held constant. In diffusion, the concentration gradient, temperature, size of the particles, and medium in which diffusion is occurring are commonly kept constant.
