Invented by Kary Mullis in 1983, the polymerase chain reaction (PCR) has grown to become a core technology in modern genetics. In genetic engineering PCR is typically used to amplify a marker for diagnostic applications or a gene of interest for insertion into an expression vector.
Polymerase chain reaction
Polymerase chain reaction
PCR
Polymerase chain reaction
The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.
Polymerase chain reaction
polymerase chain reaction
PCR stands for Polymerase Chain Reaction, a method used to amplify and copy small segments of DNA.
Nucleotides serve as the building blocks for creating new DNA strands during the polymerase chain reaction (PCR). They are incorporated by the DNA polymerase enzyme to extend the DNA strands, allowing for the amplification of specific DNA sequences.
Polymerase chain reaction
Polymerase chain reaction
Polymerase chain reaction
Polymerase chain reaction
Polymerase chain reaction
polymerase chain reaction
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
PCR