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It is true that our blood contains CO2, usually around 40mmHg, which is close to 5%. The importance in mammalian cell culture is the same as in mammalian blood. The majority of CO2 exists in the blood in the form of bicarbonate (HCO3-) which acts as a pH buffer to allow for gas, nutrient and metabolites fluctuations without causing wild pH changes. By providing a 5% CO2 overlay in a cell culture process you are ensuring that you will roughly maintain 5% dissolved CO2 (HCO3-) in solution. As cells release CO2, lactate and other pH affecting ions the CO2 concentration in solution will deviate from 5% forcing CO2 into or out of the atmosphere to maintain the balance between the two. For more information on why these systems strive to reach equilibrium research "Henry's Law."

"Our body contains 5 % CO2. So when we are culturing the cells it's needed and moisture and pH is important for cell culture it will maintain the same"

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