LB Broth allows you to create a suspension of bacterial cells from your original colony growing on an agar plate. It provides the necessary nutrients and environment for optimal replication. In suspension, bacterial cells can be plated onto more agar plates, in order to create a streak to cultivate colonies from a single cell, or used to in purification reaction in order to extract the transformed plasmid.
800ml H2O10g Bacto-tryptone5g yeast extract10g NaClAdjust pH to 7.5 with NaOHAdd 15g agarMelt agar into solution in the microwaveAdjust volume to 1L with dH2OSterilize by autoclaving
Agar in Potato dextrose agar serves as a solidifying agent to provide a gel-like consistency that allows for the growth of microorganisms. It also helps to evenly distribute nutrients throughout the medium to support the growth of fungi and bacteria.
Glucose in Plate Count Agar provides a carbon source for microbial growth. It serves as an energy source for bacteria to proliferate and form visible colonies on the agar plate.
Iodine in Czapek Dox agar is added as a source of iodine for microbial growth. It is essential for the growth of certain fungi that require iodine for metabolic processes.
Approximately 2 teaspoons of agar agar powder is equivalent to 7 grams.
function of agar slopes
800ml H2O10g Bacto-tryptone5g yeast extract10g NaClAdjust pH to 7.5 with NaOHAdd 15g agarMelt agar into solution in the microwaveAdjust volume to 1L with dH2OSterilize by autoclaving
Escherichia coli and many others
Agar is made from algae and is used as a from of Gelatin or binder. It was discovered in Japan in the 1650's.
Lactose is used as a selective nutrient in the agar as not all microbes can process lactose
Agar in Potato dextrose agar serves as a solidifying agent to provide a gel-like consistency that allows for the growth of microorganisms. It also helps to evenly distribute nutrients throughout the medium to support the growth of fungi and bacteria.
Glucose in Plate Count Agar provides a carbon source for microbial growth. It serves as an energy source for bacteria to proliferate and form visible colonies on the agar plate.
Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores
Nutrient agar plates: used for general growth of most bacteria. Blood agar plates: used to differentiate bacteria based on their ability to hemolyze red blood cells. MacConkey agar plates: used to differentiate lactose-fermenting bacteria from non-lactose fermenters based on their ability to grow and ferment lactose.
Agar-agar
Iodine in Czapek Dox agar is added as a source of iodine for microbial growth. It is essential for the growth of certain fungi that require iodine for metabolic processes.
an extract of seaweed