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What is meant by gene knockout testing?
Gene knockout testing is a technique used to study the function of a specific gene by inactivating or "knocking out" the gene from an organism's genome. This is typically achieved through genetic engineering methods such as CRISPR-Cas9 to create organisms that lack the target gene. By observing the effects of the gene knockout on the organism, researchers can learn more about the gene's normal function and its role in biological processes.
What is the normal function of the retinal blastoma gene?
tumor suppresser gene which codes for a protein that regulates E2F protein which is a cell cycle controller
How are the knockout mice useful in determining the function of genes?
Knock out mice are produced by impairing or deleting a particular gene expression. Thus the gene function (which was deleted) can be tracked by studying this knock out and the control mice. Lets say a gene is suspected to trigger immune response against a pathogen, one can study this by infecting the wild type(normal) and knock out(specific gene deleted) mice and study the role of the gene.
What is the gene expression?
Gene expression is the process by which inheritable information from a gene, such as the DNA sequence, is made into a functional gene product, such as protein or RNA.
Where is the multiple cloning site found?
The multiple cloning site is typically found within a plasmid vector, often situated within the lacZ gene of a plasmid. This site contains several unique restriction enzyme recognition sequences, allowing for the insertion of foreign DNA fragments for cloning purposes.
Where is the multiple cloning site present in pUC 18?
It is within the LacZ' gene.
What is the birth name of Marian Lacz?
Marian Lacz's birth name is Marian Mikolaj Lacz.
How tall is Laura Lacz?
Laura Lacz is 168 cm.
How tall is Marian Lacz?
Marian Lacz is 182 cm.
What nicknames did Marian Lacz go by?
Marian Lacz went by Makus.
When was Laura Lacz born?
Laura Lacz was born on October 25, 1954, in Warsaw, Mazowieckie, Poland.
When was Marian Lacz born?
Marian Lacz was born on December 5, 1921, in Rzeszw, Podkarpackie, Poland.
When did Marian Lacz die?
Marian Lacz died on August 2, 1984, in Warsaw, Mazowieckie, Poland.
Explain the principle of insertional inactivation by giving a suitable example?
insertional inactivation is basically about the inactive of second marker where the protein cannot be longer produced. the suitable example is pUC19 where the lacZ gene that encoded for beta-galactosidase cannot be longer produced when the foreign gene is inserted in it..
What is the definition of microbial genetics?
it is the study of gene and gene function in micro organisms.
What happens to the expression of the lacl gene if lactose is not available in the cell?
The expression of the lacI gene will remain active in the absence of lactose. As a repressor gene, lacI produces a protein that binds to the operator site on the lac operon, preventing RNA polymerase from transcribing the lacZ, lacY, and lacA genes. This leads to low expression of the lac operon when lactose is not present.
How is it possible for a point mutation to have no effect on the function of gene?
If the point mutation does not change the protein to be translated in the 3-letter sequence, then it will have no effect on the gene's function.
