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to vizualise DNA after Agarose gel electrophoresis

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12y ago

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What is a uv transilluminator?

A UV transilluminator is a device that uses ultraviolet light to visualize and analyze DNA or proteins separated by gel electrophoresis. It is commonly used in molecular biology laboratories to image DNA bands or protein samples that have been fluorescently tagged. UV transilluminators are essential tools for researchers to confirm successful DNA/RNA separation or protein purification processes.


Does UV light sterilize utensils?

UV light radiation kills bacteria and can sterilize utensils (UV rays are used to sterilize goggles in the laboratory, for example.


How does UV light change DNA and what are the effects of this change on genetic material?

UV light can cause changes in DNA by creating mutations, which are alterations in the genetic code. These mutations can lead to various effects on genetic material, such as cell death, cell cycle arrest, or the development of cancer. UV light can also cause DNA damage, such as the formation of thymine dimers, which can disrupt the normal functioning of DNA and lead to genetic instability.


What step was necessary to make the DNA visible?

The DNA needed to be stained with a dye, such as ethidium bromide or SYBR Green, that binds to the DNA molecules and fluoresces under ultraviolet light. This allows the DNA to become visible when viewed under a UV transilluminator or gel documentation system.


What genetic factors influence how UV affects people?

sunscreen THatZ NoT A FAcToR GEnIUz


Does UV light cause mutation?

yes uv does cause mutations by introducing distortions in the form of thymine thymine dimers


Can UV rays penetrate glass of petridish?

Yes, UV rays can penetrate glass, but the amount of UV radiation that passes through glass may depend on the type and thickness of the glass. It is generally recommended to use special UV-transmitting glass if working with UV-sensitive materials in a laboratory setting.


Can we use the UV light in laminar air flow to see DNA bands in agarose gels stained with Ethidium bromide?

One cannot use the UV light installed in a laminar air flow hood to visualize DNA in an agarose gel. You will have to use an instrument called a UV transillumunator, which illuminates the gel from below to see the stained DNA.


What genetic factors influence how uv effects people?

yea it can cause cancer around the surrounding of your skin


Can genetic mutation be sped up by ultra violet rays?

UV rays and other radiation can indeed cause mutation.


How can you use the mutation and mutagen in the same sentence?

Exposure to a mutagen, such as UV radiation, can lead to genetic mutations in organisms.


How do UV-B rays affect people?

UV light (particularly UV-B and UV-C, which are regions of the spectrum with shorter wavelengths than the UV-A part) can cause skin cancer. It does this because the DNA molecules in your body absorb UV light, and the light has enough energy to break bonds in the DNA, causing harmful rearrangements in your genetic code. When cells with damaged DNA reproduce, that can result in cancerous cells. See more details here: http://en.wikipedia.org/wiki/Ultraviolet