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To find out how enzyme concentration affects the activity of the enzyme you must:

  • vary the concentration of the enzyme, by preparing different concentrations (keeping the volume of solution the same)
  • keep the temperature, substrate concentration and pH constant
  • measure the activity of the enzyme at each concentration

How the enzyme activity is measured will depend on the specific enzyme involved.

You need to have plenty of substrate (excess substrate) so it doesn't run out during the experiment.

In this type of experiment, the enzyme activity is the dependent variable, the temperature, pH and substrate concentration are control variables and the enzyme concentration is the independent variable.

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