What is the main contribution of the scientist okazaki in genetics?

Answer 1

Okazaki discovered the way in which the lagging strand of DNA is replicated via fragments by conducting an experiment using E. coli. After reacting E. coli with 3[H] Thymidine to synthesize DNA for only ten seconds, he placed the sample in a test tube of alkaline sucrose. The larger, heavier DNA flowed to the bottom of the test tube, while the smaller lighter DNA did not. When samples were taken from the bottom of the test tube, it was found that half were heavy and half were light, proving that half of the DNA was complete and half was in fragments. Then he took a sample of E.coli DNA that had been synthesized for an additional five seconds, and found all the activity now resulted in the larger molecular weight. Therefore, there were no longer any fragments. This proved that during the five second chase, the RNA primer was removed-using DNA Polymerase I- and the bases were joined together by DNA Ligase, leaving the new DNA fully mature and repaired.

Answer 2

Okazaki's main contribution in genetics is the discovery of Okazaki fragments during DNA replication. These short, discontinuous DNA fragments are synthesized on the lagging strand of the DNA replication fork and play a crucial role in the synthesis of new DNA strands. This discovery helped unravel the mechanism of DNA replication and how genetic information is accurately transmitted during cell division.