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In coomassie staining, a dye binds to all proteins, visualizing all of them. In this way, it's nonspecific. The dye diffuses into the the original gel.
In a western, an antibody binds specifically to one or more proteins, making it specific for proteins to which the antibody can bind. In order for the antibody to access the proteins from the original gel, they are moved (blotted) to the surface of a membrane (usually nitrocellulose).
What else can I help you with?
How long does it take to stain gel?
The time it takes to stain a gel can vary depending on the type of stain used and the gel's composition. Generally, staining with common dyes like Coomassie Brilliant Blue or silver stain can take anywhere from a few minutes to several hours. After applying the stain, it's often necessary to incubate the gel for a specific period, typically ranging from 30 minutes to overnight, followed by a wash step to remove excess dye. Always refer to the specific staining protocol for the best results.
How do you think the stain changes the sample?
A stain is the result of an interaction between substances.
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
What is the difference between Iodine bought in a pharmacy and the Iodine stain solution?
The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.
How do surfactants work in stain removers?
Surfactants in stain removers work by reducing the surface tension of water, allowing it to more easily penetrate and lift stains from fabric. They help to break down the bonds between the stain molecules and the fabric, making it easier to remove the stain during the washing process.
Difference between silver stain and coomassie?
coomassie brilliant blue dye is a linear dye whereas the silver stain dye is a nonlinear one. Besides that coomassie dye has a lower sensitivity and is used for detection of highly abundant proteins. Silver stain relies on adsorption rather than absorption and is more suitable for detection of low abundant proteins but with a higher sensitivity.
What is the difference between a varnish and a stain?
A stain is used to color the wood while a varnish is a topcoat used to protect the wood.
What is the difference between Wright stain and Giemsa stain?
The Wright stain is a combination of eosin and methylene blue dyes, while Giemsa stain is a mixture of eosin, methylene blue, and azure dyes. Giemsa stain is commonly used for staining blood smears to visualize parasites and bacteria, while Wright stain is used more for general cell morphology in blood and bone marrow specimens.
What is the different between normal dirt and a stain?
The difference between normal dirt and a stain is in their difficult to wash off. Normal dirt comes off easily but a stain is a colored patch which penetrative and thus making it hard to remove.
What is the basic for gram stain results between different bacteria?
its the difference in cell wall of both the type of bacteria
What is the difference between 'dieing' and 'dying'?
The difference between Dieing and Dying is that Dieing means to stain with color. Cut, form shape with a die as in metal. Dying means passing away as in death.
How long does it take to stain gel?
The time it takes to stain a gel can vary depending on the type of stain used and the gel's composition. Generally, staining with common dyes like Coomassie Brilliant Blue or silver stain can take anywhere from a few minutes to several hours. After applying the stain, it's often necessary to incubate the gel for a specific period, typically ranging from 30 minutes to overnight, followed by a wash step to remove excess dye. Always refer to the specific staining protocol for the best results.
Which is better alkyd stain or oil based stain?
There is no difference. They are one in the same.
What are the differences between pre-stain and no pre-stain methods when it comes to wood finishing?
The main difference between pre-stain and no pre-stain methods in wood finishing is that pre-stain involves applying a special solution to the wood before staining to help the stain absorb more evenly and prevent blotching. On the other hand, the no pre-stain method skips this step and directly applies the stain to the wood surface. Pre-stain can result in a more uniform and consistent finish, while the no pre-stain method may lead to uneven staining and blotchy results.
What is the difference between ZN stain and modified ZN stain?
in Zn we use 3% decolorizer, while in modified 1% h2so4 is used, modified is used for paracite like Cryptosporidium, cyclosporidium and bacterias like nocaddia and actinomyces. also in modified heating isn't done.
What is the difference between gram-positive and gram-negative bacteria?
The presence of thick cell wall. Gram positives have a thick cell wall and remain blue. Gram negatives have a thin cell wall where the blue stain washes out and then will stain pink with the counter stain.
What is the difference between water stain and water mold?
Water mold is when something has been saturated in the water for some time and was never dried off. Water stain means something was saturated but then cleaned off to prevent mold from growing.
