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steriling the acessories by introducing saturated steam with required pressure

high temperature under the influence if high pressure that is 121 degree celsius temp and 15 ATM pressure.

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What water do you use in an autoclave?

Demineralised water should best be used in an autoclave.


How do you wrap gauze to sterilize it in the autoclave?

To wrap gauze for sterilization in an autoclave, first, place the gauze in a sterile container or on a clean, flat surface. Fold the gauze neatly to ensure it fits within the size of the autoclave tray, allowing for sufficient steam circulation. Use a sterile autoclave wrap or paper to enclose the gauze, ensuring it is secured with autoclave tape or string, which will change color after sterilization. Finally, place the wrapped gauze in the autoclave, ensuring it is positioned to allow for proper steam penetration.


How do you use a wilmot castle autoclave sterilizer?

To use a Wilmot Castle autoclave sterilizer, ensure it is plugged in and water levels are adequate. Place items inside the autoclave, close the door securely, set the appropriate temperature and pressure, and start the sterilization cycle. Once the cycle is complete, allow the autoclave to cool before opening the door and removing the sterilized items.


What is the name of the organism used to test the autoclave?

The organism commonly used to test the effectiveness of an autoclave is Bacillus stearothermophilus. This bacterium is a spore-forming organism that is highly resistant to heat, making it an ideal biological indicator for assessing sterilization processes. By placing vials containing these spores inside the autoclave, one can confirm whether the autoclave has achieved the necessary temperature and pressure for effective sterilization. After the autoclave cycle, the vials are incubated to check for any growth, indicating if the sterilization was successful.


Is it safe to autoclave a volumetric flask?

No, it is not safe to autoclave a volumetric flask as the high temperature and pressure in an autoclave can damage the flask's accuracy and calibration markings. It is recommended to clean volumetric flasks using alternative methods such as washing with detergent and disinfecting with ethanol or bleach.