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steriling the acessories by introducing saturated steam with required pressure

high temperature under the influence if high pressure that is 121 degree celsius temp and 15 ATM pressure.

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What water do you use in an autoclave?

Demineralised water should best be used in an autoclave.


How do you wrap gauze to sterilize it in the autoclave?

To wrap gauze for sterilization in an autoclave, first, place the gauze in a sterile container or on a clean, flat surface. Fold the gauze neatly to ensure it fits within the size of the autoclave tray, allowing for sufficient steam circulation. Use a sterile autoclave wrap or paper to enclose the gauze, ensuring it is secured with autoclave tape or string, which will change color after sterilization. Finally, place the wrapped gauze in the autoclave, ensuring it is positioned to allow for proper steam penetration.


How do you use a wilmot castle autoclave sterilizer?

To use a Wilmot Castle autoclave sterilizer, ensure it is plugged in and water levels are adequate. Place items inside the autoclave, close the door securely, set the appropriate temperature and pressure, and start the sterilization cycle. Once the cycle is complete, allow the autoclave to cool before opening the door and removing the sterilized items.


Is it safe to autoclave a volumetric flask?

No, it is not safe to autoclave a volumetric flask as the high temperature and pressure in an autoclave can damage the flask's accuracy and calibration markings. It is recommended to clean volumetric flasks using alternative methods such as washing with detergent and disinfecting with ethanol or bleach.


What type of water must be used in a steam autoclave?

Distilled water should be used in a steam autoclave to prevent mineral deposits and prolong the life of the equipment. Using tap water or other types of water can damage the autoclave and compromise the sterilization process.