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When conc. sulfuric acid is added to cholesterol, a water molecule is removed from C3 of cholesterol molecule, and it is oxidised to form3,5-cholestadiene.This product is converted to a polymer containing a chromophore which results in the green colouration indicating a positive test.

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What is the reaction in Lieberman's burchard test for cholesterol?

In Lieberman's burchard test for cholesterol, the reaction involves the addition of acetic anhydride and concentrated sulfuric acid to a solution containing cholesterol. This results in a color change from red to green or blue-green, indicating the presence of cholesterol.


What is involved in the Rorschach test?

The Rorschach test is a projective personality assessment based on the test taker's reaction to a series of 10 picture inkblots cards. This is a German test considered a little controversal.


What are the identification test for saponin glycoside?

Identification tests for saponin glycosides include foam test (formation of stable foam when shaken with water), hemolytic test (lysis of red blood cells), and Lieberman-Burchard test (formation of red color with acetic anhydride and concentrated sulfuric acid). Additionally, thin-layer chromatography and spectroscopic methods can be used for further confirmation of saponin glycosides.


Which is the role RNA during translation?

It's involved in making proteins. Good luck on your Biology test.


How do you field test a square d circuit breaker?

To field test a Square D circuit breaker, you can use a multimeter to check for continuity through the breaker. This involves checking if there is electrical continuity between the input and output terminals when the breaker is in the ON position. Additionally, you can test the tripping mechanism by manually triggering the overload or short-circuit protection to ensure the breaker functions properly.

Related Questions

Procedure of Lieberman Burehard test?

This is a misspelling of Liebermann-Burchard test and is probably the result of Optical Character Recognition confusing a 'c' for an 'e'.


Principles of lieberman-burchard test?

It is a test for unsaturated steroids (as cholesterol) and triteness based on the formation of a series of colors (as pink to blue to green) with acetic an-hydride in the presence of concentrated sulfuric acid called also Liebermann-Burchard test


What is the positive result and the importance of liebermann burchard and salkowski tests?

it is a test for presence of cholestrol and if two layers form, upper layer becomes red and lower one becomes yellow, it indicates the presence of cholestrol.


What is the positive and importance results in salkowski and liebermann-burchard test?

A Salkowski's test is a test for cholesterol. When concentrated sulfuric acid is added to a chloroform solution of cholesterol, the chloroform layer shows a red to blue colour and the acid layer shows a green fluorescence. This test is named after a German chemist by the name of Ernest Leopold Salkowski.


What is the positive result and the importance of liebermann-burchard and salkowski tests?

The positive result of the Liebermann-Burchard test is a blue-green coloration, indicating the presence of cholesterol. This test is important for detecting the presence of cholesterol in biological samples, which can be indicative of certain diseases or conditions. The positive result of the Salkowski test is the formation of a red color when testing for the presence of sterols, including cholesterol. This test is valuable in identifying the presence of sterols in samples such as plant extracts or microbiological cultures, aiding in research and diagnostic purposes.


What is the reaction in Lieberman's burchard test for cholesterol?

In Lieberman's burchard test for cholesterol, the reaction involves the addition of acetic anhydride and concentrated sulfuric acid to a solution containing cholesterol. This results in a color change from red to green or blue-green, indicating the presence of cholesterol.


Does testosterone gives a positive results with lieberman-burchard test?

Testosterone can have an impact on aggression and risk-taking behavior, which may influence responses on the Lieberman-Burchard test. However, the test is designed to measure personality traits, so the results can be influenced by various factors besides testosterone levels. It is important to consider all aspects of an individual's behavior and not solely focus on hormonal influences when interpreting the test results.


What is the mechanism of formaline test?

What is the mechanism of formaline test


Do I pay for a dna test when there’s a court order is involved?

Do I pay for a dna test when there’s a court order is involved?


Why is the term 'test tube baby' NOT accurate?

There are no test tubes involved.


What is the test to determine the existence of a partnership?

The test to determine the existence of a partnership is in the conduct of the partners involved. The real intentions of the parties involved in a partnership are often used as the true test in determining the existence of the partnership.


What are some advantages of isotopic tracers?

Isotopic tracers can be used to take the place of an atom that is part of a molecule with known structure. You can then react that molecule with another and test your sample for your isotope. This allows you to determine the reaction mechanism because you know if your specific atom was involved.