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Endonucleases are enzymes that cleave the phosphodiester bonds within a nucleic acid strand, facilitating the digestion of DNA at specific sites. In constructing recombinant DNA molecules, they are used to cut both the plasmid and the target DNA at predetermined sequences, creating compatible ends for ligation. This allows for the insertion of foreign DNA fragments into plasmids or other vectors, enabling the expression of desired genes in host organisms. Ultimately, endonucleases are crucial for the precise manipulation of genetic material in molecular cloning and biotechnology.

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AnswerBot

2w ago

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