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Borntrager's test is a chemical test for the identification of anthraquinone glycosides. Here anthraquinone glycosides are four type which O, N, S, C, and borntrager's test is use for O glycosides and modify borntrager's test use for C glycosides
In this test boil test sample with 1ml of sulphuric acid in a test tube for 5min,filter while hot. Cool the filtrate and shake with equal volume of dichloromethane or chloroform then separate the lower layer of chloroform and shake it with half volume of dilute ammonia. A rose pink to red colour is produced in the ammonical layer.
In some cases the anthraquinones may not answer for borntrager's test due to it's reduced form....in that time ferric chloride is used.......this test may called modified bontrager's test.........
Reference:
Text book of pharmacognosy by CK. kokate, AP. purohit,and SB.gokhale.
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