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Light entering a solution may pass entirely through or be absorbed partially or entirely depending on the solution concentration and the wavelength of the light. When this light is absorbed it transfers energy to the medium and can only hold the excess energy for about 10-5 seconds before it must release it to fall back into its normal energy state. The difference between the maximum wavelengths of the light entering the solution and the light exiting the solution is called a Stokes Shift and is representative of a specific molecule.
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What is the principle of fluorescence?
The principle of fluorescence spectroscopy is the interaction with light image.
Which radiation is used in fluorescence spectroscopy?
Ultraviolet Electromagnetic Radiation
Could the presence of different pigments be detected before separation by chromatography?
Yes, the presence of different pigments can be detected before separation by chromatography through techniques such as UV-Vis spectroscopy or fluorescence spectroscopy. These techniques can provide information about the absorption or emission properties of the pigments present in a sample.
WHAT FRANK-condon principle says with respect to spectroscopy?
The Franck-Condon principle states that in a molecular electronic transition, the nuclei of the molecules will remain in the same vibrational state before and after the transition. This principle is important in spectroscopy as it explains the intensity and shape of spectral lines. It allows for the determination of relative energies of electronic states and can provide insight into molecular structure and bonding.
What is the peak at 800nm in fluorescence spectroscopy?
It depends what you used as your excitation wavelength. If you used 800 nm as your excitation wavelength, this is due to Rayleigh scattering, where photons from the emission source are scattered off of the molecules in your sample and are picked up by the detector. If your wavelength is shorter (like 400 nm) then this is due to Raman Scattering, where the molecule either absorbs or donates energy from/to the photon during the scattering process. Scattering peaks are traditionally much sharper than fluorescence peaks.
What is the principle of fluorescence?
The principle of fluorescence spectroscopy is the interaction with light image.
How is fluorescence spectroscopy done?
Fluorescence spectroscopy is a type of spectroscopy that analyzes fluorescence from a provided sample. This uses a beam of light, often an ultraviolet light which then causes absorption spectroscopy to occur.
What has the author Stephen G Schulman written?
Stephen G. Schulman has written: 'Fluorescence and phosphorescence spectroscopy' -- subject(s): Fluorescence spectroscopy, Phosphorescence spectroscopy 'Molecular Luminescence Spectroscopy'
What are the uses of fluorometry?
Fluorescence spectroscopy (a.k.a. fluorometry or spectrofluorometry) is a type of electromagnetic spectroscopy which analyzes fluorescence from a sample. Fluorescence spectrocopy is used in biochemical, medical, and chemical research fields for analyzing organic compounds. Atomic Fluorescence Spectroscopy (AFS) techniques are useful in other kinds of analysis/measurement of a compound present in air or water, or other media.
Which radiation is used in fluorescence spectroscopy?
Ultraviolet Electromagnetic Radiation
What has the author Bernard Valeur written?
Bernard Valeur has written: 'Molecular fluorescence' -- subject(s): Fluorescence spectroscopy
What is Relative Fluorescence Intensity?
Relative fluorescence intensity is a measure of the amount of fluorescence emitted by a sample compared to a reference sample. It is often used in fluorescence spectroscopy to quantify the fluorescence signal from a sample relative to a standard for comparison and analysis.
What has the author Russell H Barnes written?
Russell H Barnes has written: 'Laser spectroscopy for continuous combustion applications' -- subject(s): Raman spectroscopy, Fluorescence spectroscopy, Laser spectroscopy
How does the Stern-Volmer plot illustrate the phenomenon of quenching in fluorescence spectroscopy?
The Stern-Volmer plot shows how the fluorescence intensity of a substance decreases when it is exposed to a quenching agent. This illustrates the phenomenon of quenching in fluorescence spectroscopy, where the quencher molecule reduces the fluorescence emission of the sample by either absorbing the excitation energy or deactivating the excited state of the fluorophore.
What has the author Bohdan Dziunikowski written?
Bohdan Dziunikowski has written: 'Podstawy rentgenowskiej radioizotopowej analizy fluorescencyjnej' -- subject(s): Fluorescence spectroscopy, X-ray spectroscopy
What is the relationship between excitation and emission in fluorescence spectroscopy?
In fluorescence spectroscopy, excitation is the process of stimulating a molecule to absorb light energy, causing it to move to a higher energy state. Emission is the subsequent release of this absorbed energy in the form of light. The relationship between excitation and emission is that excitation triggers emission, with the emitted light having a longer wavelength than the absorbed light. This phenomenon is used in fluorescence spectroscopy to analyze the properties of molecules and materials.
What has the author Douglas B Yager written?
Douglas B Yager has written: 'SUPERXAP manual' -- subject(s): Computer programs, Fluorescence spectroscopy, X-ray spectroscopy
