Ampicillin is added to NA agar medium to inhibit the growth of bacteria that do not contain the ampicillin resistance gene. This ensures that only bacteria transformed with a plasmid containing the resistance gene will be able to grow on the agar, allowing for selection of transformed bacteria.
rose bengal inhibits growth of bacteria, thus promoting the growth of fungi, mold and yeast.
To select for a recombinant cell that is lysine (lys) plus arginine (arg) plus ampicillin (amp)-resistant, you would add lysine and arginine to the glucose minimal salts agar as supplements to support the growth of the auxotrophic strains that cannot synthesize these amino acids. Additionally, you would incorporate ampicillin to the agar to ensure that only the cells containing the ampicillin resistance gene can grow. This combination allows for the selection of cells that are capable of synthesizing lysine and arginine and are resistant to ampicillin.
Rose Bengal agar is used for the selective isolation of fungi, particularly Cryptococcus species. Rose Bengal inhibits bacterial growth while allowing fungal growth. Streptomycin solution is added to the medium as an antimicrobial agent to further inhibit bacterial growth and enhance the selection of fungal colonies.
To make it semi-solid
A concentration of 0.5-0.7% agar is typically used in semisolid medium for motility. This is lower than the typical 1.5-2% agar concentration used in solid medium, allowing motile bacteria to move more easily through the softer semisolid medium.
rose bengal inhibits growth of bacteria, thus promoting the growth of fungi, mold and yeast.
simply agar medium
Rose Bengal agar is used for the selective isolation of fungi, particularly Cryptococcus species. Rose Bengal inhibits bacterial growth while allowing fungal growth. Streptomycin solution is added to the medium as an antimicrobial agent to further inhibit bacterial growth and enhance the selection of fungal colonies.
Differential medium.
To make it semi-solid
It is a medium that support the growth of cells or microorganisms. It is usually a liquid or a gel and there are different agar mediums for different types of cells.
To determine how much agar is needed for 300 ml of medium, we can use a proportion based on the agar quantity provided for 1 liter. 6.4 g agar is used in 1 liter, which means for 300 ml, we can calculate: (6.4 g / 1000 ml) * 300 ml = 1.92 g of agar. Therefore, 1.92 g of agar is needed for 300 ml of the medium.
marine red algae
pink
A concentration of 0.5-0.7% agar is typically used in semisolid medium for motility. This is lower than the typical 1.5-2% agar concentration used in solid medium, allowing motile bacteria to move more easily through the softer semisolid medium.
In our lab we use LB agar, LB broth, and 2XYT broth.
Semi-solid agar media have been successfully used for primary culture and drug sensitivity tests of tubercle bacilli directly from sputum homogenates. I really wanna have sex with my friend but i don't know how to start!