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Chelex is a resin used in molecular Biology to bind metal ions and remove them from a solution, particularly for DNA and RNA isolation procedures. It helps in purifying nucleic acids by chelating metal ions that can inhibit enzymatic reactions.

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1y ago

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What is the role chelex?

Chelex is a chelating resin often used in molecular biology to bind and remove metal ions from a sample. It is commonly used for DNA extraction to inactivate nucleases and stabilize DNA. Chelex can also be used in protein purification to remove metal contaminants.


What is the role chelex-100 in DNA extraction?

Chelex-100 is a chelating resin used in DNA extraction to bind and remove metal ions that can degrade DNA samples. It helps protect the DNA from degradation and improve the yield of high-quality DNA for downstream applications like PCR. Chelex-100 works by chelating divalent metal ions such as Mg2+ and other contaminants, preserving the integrity of the DNA.


What is chelex-100?

Chelex-100 is a chelating resin primarily composed of polystyrene divinylbenzene that is used to bind and remove metal ions from solutions. It is commonly employed in molecular biology and biochemistry for DNA extraction, particularly from biological samples, as it helps to purify nucleic acids by sequestering contaminants such as divalent metal ions that can degrade DNA. This resin facilitates a simple and effective method for isolating DNA while minimizing enzymatic degradation.


What mesh particle size for chelex 100 is best for DNA extraction?

A mesh particle size of 50-100 μm is commonly recommended for the use of Chelex 100 resin in DNA extraction procedures. This size allows for efficient binding of DNA while minimizing binding of larger molecules like proteins and polysaccharides.


What is the purpose of edsa revolution?

the purpose of this is to have a purpose


Use the word purpose in a sentence?

What is the purpose of this sentence?I did not do that on purpose.


What is a best sentence for purpose?

Opinions vary as to the purpose of the best sentence for the use of the word 'purpose'. This serves no purpose. I reached out to the porpoise on purpose.


What is the difference between the general purpose and the specific purpose of a speech?

The general purpose is the overall intent of your speech. The specific purpose is a goal stated in a complete sentence. Overall, the general purpose and specific purpose are different because they both mean different things. The specific purpose is just really a goal stated in your sentence and the general purpose is just the purpose of your speech.GENERAL PURPOSE: purpose of your speech.SPECIFIC PURPOSE: the goal or the main idea in your speech.


What is aesthetic purpose?

aesthetic purpose is the purpose of the asthetical question or answer


What is by purpose?

A by-purpose is an indirect or incidental purpose for an object or an action.


What is a by-purpose?

A by-purpose is an indirect or incidental purpose for an object or an action.


What is the meaning of purpose if purpose has no meaning?

That is a trick question and purpose does have a meaning.If purpose has no meaning, then the meaning of purpose is the subjective experience of seeking a reason to defend the ego against assault.this is very complicated______Hey, I may add this to the guy up there.What's the purpose of breathing?To live.What's the purpose of playing?To have fun.What's the purpose of living?To make a difference.What's the purpose of purpose?To have a reason. To have an opinion.~Chelseam2------------------------------------------------------------------There is no purpose that has no meaning.