Extracellular enzyme is an enzyme that performs its role or function outside a cell. The purpose of experimenting extracellular enzyme is to know how can it affect our body when the bacteria secretes.
No, pepsin is not the substrate in the experiment with BAPNA. BAPNA is the synthetic substrate used in this experiment to test the activity of the enzyme pepsin by measuring the rate of substrate cleavage. Pepsin acts on BAPNA as the enzyme, not the substrate.
the purpose is how is is getting bigger
Extracellular enzymes are enzymes that work outside of the cell that produce them. Amylase is an enzyme that breaks down starch into simpler sugars like glucose. Amylase is commonly found in saliva and pancreatic secretions to aid in the digestion of carbohydrates.
To test the hypothesis that an enzyme is not used up during a reaction, you can perform a simple experiment where you measure the enzyme activity before and after the reaction. If the enzyme activity remains the same before and after the reaction, it indicates that the enzyme is not used up. This can be done by measuring the substrate conversion rate or product formation rate.
Sucrase is an enzyme which catalyze the hydrolysis of sucrose to fructose and glucose.
Intracellular enzymes are enzymes that function inside the cell, where they participate in various metabolic reactions. Extracellular enzymes, on the other hand, are enzymes that are secreted outside the cell to catalyze reactions in the extracellular environment, such as breaking down larger molecules into smaller ones for nutrient absorption.
An extracellular enzyme is one which reacts outside of the cell. An intracellular enzyme is one which reacts inside of the cell.
Extracellular enzyme activity is easier to determine because extracellular enzymes are released outside the cell and can be directly measured in the surrounding environment. In contrast, intracellular enzymes are contained within the cell, requiring cell disruption to access them for measurement, which can introduce variability and complexity to the assay.
No, pepsin is not the substrate in the experiment with BAPNA. BAPNA is the synthetic substrate used in this experiment to test the activity of the enzyme pepsin by measuring the rate of substrate cleavage. Pepsin acts on BAPNA as the enzyme, not the substrate.
the purpose of doing an experiment is to discover new things
The amount of amylase enzyme needed for the experiment should be determined based on the specific protocol or instructions provided. It is important to follow the guidelines accurately to ensure the success of the experiment.
The factor in an experiment that is changed on purpose is called the independent variable. This variable is manipulated by the researcher to observe its effect on the dependent variable. It is the cause being studied in the experiment.
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materials and methods
Amylase is an enzyme in your saliva which can break down starch to sugar in a a matter of minutes or seconds. The salivary glands in your mouth release the amylase to break down the starch, and therefore, amylase is an extracellular enzyme.
Using a lower enzyme concentration would result in a slower rate of reaction because there are fewer enzymes available to catalyze the reaction. If the experiment did not work as expected, increasing the enzyme concentration could have potentially sped up the reaction and led to the expected results.
Be clear about the purpose of the experiment.