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HBC Buffer, or HEPES-Buffered Saline, is used in biological and biochemical applications to maintain a stable pH during experiments. It provides a consistent environment for cellular processes by buffering against fluctuations in pH, particularly during cell culture or enzyme reactions. Additionally, HBC Buffer can help preserve the activity of proteins and other biomolecules, ensuring reliable experimental results.

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6mo ago

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Use of HBC buffer in DNA extraction?

HBC buffer, which typically contains components like high salt (e.g., sodium chloride) and a detergent, is used in DNA extraction protocols to facilitate the lysis of cells and the release of DNA. The high salt concentration helps to stabilize the DNA while the detergent breaks down cellular membranes and proteins. This combination enhances the efficiency of DNA precipitation and purification, allowing for the effective separation of DNA from proteins and other cellular debris. Ultimately, using HBC buffer contributes to obtaining high-quality DNA suitable for downstream applications.


How did the old hbc retain control over the new hbc after the merger with the nwc?

HBC kept control by controlling the trade routes, which brought a lot of money into the HBC.


What the role of sds in buffer?

to disrupt cell membranes


When the hbc and the nwc merged why did they keep the hbc's name?

Because HBC was the more powerful one, either way it's now called "The Bay."


Why did the NWC and the HBC merge?

the hbc had alot of hot women and the nwc thought if they merged witht he hbc thy could maybe have some sexy time


Who owns the the HBC?

The initials HBC usually means, in Canada, the Hudson's Bay Company.


When was HBC opened?

HBC (The Hudson's Bay Company) was founded in 1670 by King Charles II.


Who are the owners of hbc?

The initials HBC usually means, in Canada, the Hudson's Bay Company.


Why is HBC important?

T


What does hbc sell today?

Hbc, sell anything from clothes, dishes, bedding, curtain and much much more


Why was the HBC so important?

The Hudson's Bay Company (HBC), established in 1670, was crucial for several reasons. It played a significant role in the fur trade, establishing trade networks that connected Indigenous peoples with European markets. HBC's activities also contributed to the exploration and mapping of Canada, influencing territorial claims and settlement patterns. Additionally, it fostered economic development and laid the groundwork for Canada's eventual emergence as a nation.


What is the role of glycerol in the lysis buffer?

To protect protein during thawing and freezing