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It is true that our blood contains CO2, usually around 40mmHg, which is close to 5%. The importance in mammalian cell culture is the same as in mammalian blood. The majority of CO2 exists in the blood in the form of bicarbonate (HCO3-) which acts as a pH buffer to allow for gas, nutrient and metabolites fluctuations without causing wild pH changes. By providing a 5% CO2 overlay in a cell culture process you are ensuring that you will roughly maintain 5% dissolved CO2 (HCO3-) in solution. As cells release CO2, lactate and other pH affecting ions the CO2 concentration in solution will deviate from 5% forcing CO2 into or out of the atmosphere to maintain the balance between the two. For more information on why these systems strive to reach equilibrium research "Henry's Law."

"Our body contains 5 % CO2. So when we are culturing the cells it's needed and moisture and pH is important for cell culture it will maintain the same"

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13y ago
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9y ago

The role of oxygen in animals cells its to aid in metabolizing the glucose and glutamine for energy. The concave shape of red blood cells develop for the sol purpose of carrying oxygen to the cells.

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oxygen helps in growing the cells , producing desired product

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It transports proteins

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Q: What is the function of co2 in cell culture?
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