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A second order neuron is the 2nd neuron to carry an order. The order could be a sensory stimulus or a motor stimulus. For e.g. in order to move a muscle, the order for that muscle to contract starts at the cerebral cortex in the somatomotor area. That is the first order neuron. The axon of the 1st order neuron will synapse with the 2nd order neuron at the level of the brain stem, which commonly decussate (crosses over) to the opposite side. In turn, the axon of the 2nd order neuron will descend along the spinal cord tracts where it will synapse with the 3rd order neuron. The 3rd order neuron is located in the ventral horn of the spinal cord, which will exit with the spinal nerve to supply the muscle.

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