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What are the different types of chromatography?
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
What is the name of the method used to separate dyes?
Chromatography is the method used to separate dyes by allowing the components to move at different rates through a medium, such as paper or a column, based on their affinity for the medium and solvent. This technique separates the different dyes based on their molecular interactions with the moving phase.
Why do the component of a mixture in thin layer chromatography separate?
The components in a mixture separate in thin layer chromatography due to differences in their affinity for the stationary phase (typically a silica gel plate) and the mobile phase (solvent). Components with strong affinity for the stationary phase will move more slowly, leading to separation based on their differing interaction strengths.
What is the moving solvent called in chromatography?
The moving solvent in chromatography is referred to as the mobile phase. It carries the sample through the stationary phase, allowing for separation based on differences in affinity between the components of the sample.
Procedures that is used to separate different types of molecules in a liquid mixture?
Chromatography is a technique used to separate different types of molecules based on their size, charge, or affinity for a stationary phase in the mixture. This can be done using methods like paper chromatography, thin layer chromatography, or high-performance liquid chromatography (HPLC) depending on the specific separation requirements of the molecules.
What type of chromatography will you use to isolate mitochondrial protein?
affinity chromotography
What has the author William H Scouten written?
William H. Scouten has written: 'Affinity chromatography' -- subject(s): Affinity chromatography, Biochemistry, Technique
Is Immunoabsorption or immunoadsorption the same thing as affinity chromatography?
No, but they all rely on affinity of the substrate for a target molecule
Why some substances in chromatography will travel futher up the paper?
Substances travel further up the paper in chromatography due to differences in their affinity to the mobile phase (solvent) and the stationary phase (paper). Substances that have higher affinity for the solvent will move faster and farther up the paper, while those with higher affinity for the stationary phase will travel slower and remain closer to the origin.
What are the different types of chromatography?
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
What is the name of the method used to separate dyes?
Chromatography is the method used to separate dyes by allowing the components to move at different rates through a medium, such as paper or a column, based on their affinity for the medium and solvent. This technique separates the different dyes based on their molecular interactions with the moving phase.
Why do the component of a mixture in thin layer chromatography separate?
The components in a mixture separate in thin layer chromatography due to differences in their affinity for the stationary phase (typically a silica gel plate) and the mobile phase (solvent). Components with strong affinity for the stationary phase will move more slowly, leading to separation based on their differing interaction strengths.
What is the moving solvent called in chromatography?
The moving solvent in chromatography is referred to as the mobile phase. It carries the sample through the stationary phase, allowing for separation based on differences in affinity between the components of the sample.
Procedures that is used to separate different types of molecules in a liquid mixture?
Chromatography is a technique used to separate different types of molecules based on their size, charge, or affinity for a stationary phase in the mixture. This can be done using methods like paper chromatography, thin layer chromatography, or high-performance liquid chromatography (HPLC) depending on the specific separation requirements of the molecules.
What is the order in which compounds elute in column chromatography, with a focus on which compound typically elutes first?
In column chromatography, compounds elute based on their affinity for the stationary phase. Typically, compounds with weaker interactions with the stationary phase elute first, followed by those with stronger interactions. The compound that typically elutes first is the one with the least affinity for the stationary phase.
Why does carotene travel farthest in chromatography of leaf pigments?
Carotene travels the farthest in chromatography of leaf pigments because it is the least soluble in the chromatography solvent. This means it interacts less with the solvent and more with the chromatography paper, allowing it to move further up the paper before the solvent front stops it.
What is mobile and stationary phase in Chromatography?
In chromatography, the mobile phase is the solvent that carries the sample through the stationary phase. The stationary phase is the material that interacts with the components of the sample, causing separation based on differences in their affinity for the stationary phase.
