What precautions are taken to excessive pH changes during experiment with isolated muscle?

Answer 1

To prevent excessive pH changes during experiments with isolated muscle, buffers such as HEPES or phosphate buffers are often used to maintain a stable pH. These buffers help resist changes in pH by absorbing excess hydrogen ions or hydroxide ions. Additionally, the temperature of the solution is carefully controlled as temperature can affect the dissociation constants of the buffering agents and impact pH stability. Regular monitoring and adjustment of the pH levels throughout the experiment are also crucial to ensure accurate and reliable results.

Answer 2

Well, honey, to prevent pH changes from going off the charts during an experiment with isolated muscle, you gotta keep things in check by using a buffer solution. This bad boy acts like a bodyguard, making sure the pH levels stay stable and don't go haywire. So, don't forget to have that buffer solution on standby to keep your isolated muscle experiment running smoothly.