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The scientific field that uses recombinant DNA is called genetic engineering.

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What scientific was field was created when recombinant DNA was first made in the 1970s?

genetic engineering


What occurs first in the production of a recombinant plasmid?

In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.


How can you get the scientific world to accept your discovery?

To get the scientific world to accept your discovery, you should first publish your findings in reputable scientific journals, present your work at conferences, and engage with other researchers in the field to gather feedback and support. Replicability of your results by other scientists is key to gaining credibility and acceptance in the scientific community.


Who discovered recombinant DNA technology?

Recombinant DNA technology was developed in the early 1970s by Paul Berg, who is often credited with its discovery. His groundbreaking work involved the combination of DNA from different sources, leading to the creation of the first recombinant DNA molecules. This innovation paved the way for significant advancements in genetic engineering and biotechnology. Other key figures in the development of this technology include Herbert Boyer and Stanley Cohen, who further refined the techniques for cloning and manipulating DNA.


How did Boyer and Cohen performe their recombinant DNA experiment?

Herbert Boyer and Stanley Cohen created the first recombinant DNA organism using recombinant DNA technology, or gene splicing, which allows the manipulation of DNA. They showed that the gene for a frog ribosomal RNA could be transferred and expressed in bacterial cells. Boyer and Cohen removed plasmids, small rings of DNA located in a cell's cytoplasm, from a cell. Using restriction enzymes, they cut the DNA at precise positions and then recombined the DNA strands in their own way using DNA ligase enzyme. They then inserted the altered DNA into E. coli bacteria. The bacterial cells could be made to produce specific proteins using gene splicing. This technology was a major breakthrough for genetic engineering. Their experiments dramatically demonstrated the potential impact of DNA recombinant engineering on medicine and pharmacology, industry and agriculture.

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