Observations by Erwin Chargaff that concentrations of the four nucleotide bases differ among species. Within a species, the concentrations of adenine and thymine are always about the same and the concentrations of cytosine and guanine are always about the same. In each species, the amount of adenine equals the amount of thymine. The amount of guanine equals the amount of cytosine.
base pairing rules
Chargaff’s rules provided Watson and Crick with crucial information about the base pairing in DNA: adenine pairs with thymine and guanine pairs with cytosine in equal amounts. This information helped them to propose the double helix structure of DNA, with complementary base pairing along the strands.
Chargaff's rule means that there should be the same number of purine and pyrimidine bases in DNA. The base pairing rules means that A always pairs with T, and G always pairs with C ie. a purine always pairs with a pyrimidine, so there must be the same number of both proving Chargaff's rule.
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Watson and Crick's model of DNA structure, the double helix, provided a physical explanation for Chargaff's rules by showing how the complementary base pairing of adenine-thymine and guanine-cytosine could fit within the double helix structure. This pairing resulted in equal amounts of A and T, and G and C, in a DNA molecule, which aligned with Chargaff's observation that the amounts of adenine and thymine, and guanine and cytosine, were nearly equal in DNA samples.
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base pairing rules
0.000537 = 5.37 x 10^-4
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According to Chargaff's rules, the amount of adenine always equals the amount of thymine in a DNA molecule. Similarly, the amount of guanine always equals the amount of cytosine. This principle is fundamental to the structure of DNA and base pairing.
Chargaff's rule means that there should be the same number of purine and pyrimidine bases in DNA. The base pairing rules means that A always pairs with T, and G always pairs with C ie. a purine always pairs with a pyrimidine, so there must be the same number of both proving Chargaff's rule.
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Thymine is always paired with adenine in a DNA molecule according to Chargaff's rules. They form a complementary base pair, with adenine pairing with thymine through two hydrogen bonds.
A positive statement is followed by a negative tagA negative statement is followed by a negative tag
Chargaff’s rules provided Watson and Crick with crucial information about the base pairing in DNA: adenine pairs with thymine and guanine pairs with cytosine in equal amounts. This information helped them to propose the double helix structure of DNA, with complementary base pairing along the strands.
One would restate a financial statement is, for example, new information comes to light after the financial statement was first produced. Sometimes accounting rules change and to allow comparison with accounts done under the new rules, previous accounts are restated to comply with the new rules.