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What is locating agent used for?

Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.


What are the two techniques used in paper chromatography to identify caffeine in tea?

The two techniques used in paper chromatography to identify caffeine in tea are stationary phase and mobile phase. In stationary phase, a sheet of filter paper is used to hold the sample, while in mobile phase, a solvent is used to carry the sample along the paper. These techniques separate the components of the sample based on their affinity for the stationary and mobile phases.


What is The appearance of chromatograms in paper chromatography?

The chromatogram in paper chromatography is just the paper itself. You can look at the paper and see the dots that have risen due to the solvent. The appearance is just simply a piece of paper with dots that have risen from the baseline to a certain spot on the paper. see related link below for more info


How do you obtain chromatogram?

A chromatogram is obtained by running a sample through a chromatography technique such as gas chromatography (GC) or liquid chromatography (LC). The components of the sample separate based on their unique properties as they move through the stationary phase in the column. Detection methods such as mass spectrometry or ultraviolet-visible spectroscopy are then used to generate a chromatogram showing the peaks corresponding to each component.


Why is a chromatogram like a chemical fingerprint?

A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.

Related Questions

What is locating agent used for?

Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.


Describe different consultation techniques used to identify treatment objectives?

Describe different consultation techniques used to identify waxing treatment objectives


What are the two techniques used in paper chromatography to identify caffeine in tea?

The two techniques used in paper chromatography to identify caffeine in tea are stationary phase and mobile phase. In stationary phase, a sheet of filter paper is used to hold the sample, while in mobile phase, a solvent is used to carry the sample along the paper. These techniques separate the components of the sample based on their affinity for the stationary and mobile phases.


What are the distinguishing characteristics of flies with spots on their wings?

Flies with spots on their wings have distinct markings that help differentiate them from other fly species. These spots can vary in size, shape, and color, and are often used by scientists and researchers to identify and classify different types of flies.


What is base peak in hplc?

The base peak in HPLC refers to the peak in the chromatogram that corresponds to the highest intensity or abundance of ions detected by the mass spectrometer. It is used as a reference peak for quantification and identification of other peaks in the chromatogram.


What is The appearance of chromatograms in paper chromatography?

The chromatogram in paper chromatography is just the paper itself. You can look at the paper and see the dots that have risen due to the solvent. The appearance is just simply a piece of paper with dots that have risen from the baseline to a certain spot on the paper. see related link below for more info


How do you obtain chromatogram?

A chromatogram is obtained by running a sample through a chromatography technique such as gas chromatography (GC) or liquid chromatography (LC). The components of the sample separate based on their unique properties as they move through the stationary phase in the column. Detection methods such as mass spectrometry or ultraviolet-visible spectroscopy are then used to generate a chromatogram showing the peaks corresponding to each component.


Chromatograghy in hand written letters?

Chromatography is a technique used to separate and analyze mixtures of substances into their individual components. It works by allowing a sample to move through a stationary phase at different rates based on their interactions with the stationary phase. This separation can be visualized by the different bands or spots created on the chromatogram.


Why is a chromatogram like a chemical fingerprint?

A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.


What is the purpose of using ninhydrin as a TLC stain in chromatography experiments?

Ninhydrin is used as a TLC stain in chromatography experiments to detect and visualize amino acids and other compounds that contain primary amines. It reacts with these compounds to form a colored product, making it easier to identify and analyze them on the chromatogram.


What was one technique used to identify the double helix?

X-ray crystallography was one of the main techniques. The arrangement of atoms was worked out from the patterns obtained.


How can one effectively interpret an HPLC chromatogram?

To effectively interpret an HPLC chromatogram, one must analyze the peaks in the graph to determine the retention times, peak shapes, and peak heights of the compounds being separated. By comparing these characteristics to known standards or reference materials, one can identify and quantify the compounds present in the sample. Additionally, understanding the mobile phase composition, column properties, and detector settings used in the HPLC analysis can provide valuable insights into the separation process.