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Why do you use buffers in separation of serum proteins by electrophoresis?
Buffers are used in electrophoresis to maintain a constant pH, which helps to separate serum proteins based on their charge. By keeping the pH stable, the proteins retain their charges and migrate towards the electrodes at different rates, allowing for separation based on size and charge.Buffers also help maintain an optimal environment for the proteins to remain stable and active during the electrophoresis process.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What are inert and active electrodes?
Inert electrodes simply serve as electrical conductors and are unchanged by the cell processes. Active electrodes change during the cell reactions. An example of inert electrodes is platinum in the electrolysis of water. The platinum remains unchanged and the water is split into hydrogen and oxygen. An example of active electrodes is in the electrolysis of copper sulfate solution with copper electrodes. The anode copper is converted into copper ions in the solution, and metallic copper builds up on the cathode. The term active electrode is also used in eeg measurement, here as the opposite to passive electrode. Electrodes are the pads attached to the skin to get readings, and active ones don't need a conductive paste to get a good signal.
Why is glycerol and blue dye added to the gel loading buffer during gel electrophoresis?
Glycerol is added to make the DNA sample denser so that it sinks into the gel and loads properly. Blue dye is added to visualize the sample loading and migration progress during electrophoresis.
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
Why do you use buffers in separation of serum proteins by electrophoresis?
Buffers are used in electrophoresis to maintain a constant pH, which helps to separate serum proteins based on their charge. By keeping the pH stable, the proteins retain their charges and migrate towards the electrodes at different rates, allowing for separation based on size and charge.Buffers also help maintain an optimal environment for the proteins to remain stable and active during the electrophoresis process.
Which pieces of DNA will migrate to the bottom of the DNA gel first during electrophoresis?
During electrophoresis, smaller pieces of DNA will migrate to the bottom of the gel first.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the function of comb in electrophoresis?
The comb is used to create wells in the gel where samples can be loaded for electrophoresis. It helps to organize the samples and ensure that they are separated properly during the process.
Where do you place the DNA samples on the gel during electrophoresis?
During electrophoresis, DNA samples are placed at the wells of the gel. The gel is then subjected to an electric current, causing the DNA fragments to move through the gel based on their size.
Why do DNA pieces migrate from the top of the gel towards the bottom during gel electrophoresis?
During gel electrophoresis, DNA pieces migrate from the top of the gel towards the bottom because they are negatively charged and are attracted to the positive electrode at the bottom of the gel.
What are ignition electrodes on boilers?
IGNITION ELECTRODES IMPLIES TWO ARE INSTALLED. TWO ELECTRODES IMPLIES AN OIL FIRED BOILER. THE ELECTRODES ARE TIED ELECTRICALLY TO THE SECONDARY WINDINGS OF A HIGH VOLTAGE TRANSFORMER. DURING IGNITION THE TRANSFORMER PRODUCES A SPARK BETWEEN THE ELECTRODES TO IGNITE THE ATOMIZED MIXTURE THAT THE BURNER MOTOR AND OIL PUMP PRODUCE AND DELIVER VIA THE NOZZLE. LC
What are inert and active electrodes?
Inert electrodes simply serve as electrical conductors and are unchanged by the cell processes. Active electrodes change during the cell reactions. An example of inert electrodes is platinum in the electrolysis of water. The platinum remains unchanged and the water is split into hydrogen and oxygen. An example of active electrodes is in the electrolysis of copper sulfate solution with copper electrodes. The anode copper is converted into copper ions in the solution, and metallic copper builds up on the cathode. The term active electrode is also used in eeg measurement, here as the opposite to passive electrode. Electrodes are the pads attached to the skin to get readings, and active ones don't need a conductive paste to get a good signal.
Does the Boeing 777 have outlets for passengers to charge their devices during the flight?
Yes, the Boeing 777 typically has outlets available for passengers to charge their devices during the flight.
Why does DNA move through the gel during gel electrophoresis?
During gel electrophoresis, DNA moves through the gel because it is negatively charged and is attracted to the positive electrode. The DNA molecules are pulled through the gel by an electric field, separating them based on size.
Can always keeping pc plugged in during non use damage my computer?
Yes, by keeping the PC plugged in during the non-use can really damage the computer.
How can I effectively tile around outlets in my home during a renovation project?
To effectively tile around outlets during a renovation project, you can use outlet extenders to bring the outlets forward to be flush with the new tile. This will ensure a clean and professional finish.
