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A Y-linked gene is a gene that is carried on the Y chromosome, a sex chromosome found only in male cells. In humans, the most commonly mentioned Y-linked gene encodes a protein called testis-determining factor (TDF). As the name suggests, TDF prompts the development of the testes. Since only genetic males have a Y chromosome, only males express TDF and go on to develop testes.

In general, genes carried on the sex chromosomes (X and Y) are considered to be "linked" to that chromosome. Genes carried on the X chromosome are considered X-linked in the same way that TDF is considered Y-linked.

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