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Ribonuclease is primarily secreted by the pancreas into the small intestine. It plays a crucial role in the digestion of RNA by breaking down ribonucleic acid into smaller components. Additionally, ribonuclease can also be found in other tissues and secretions, such as saliva and certain immune cells, where it may contribute to various biological processes.

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5mo ago

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Why does ribonuclease become inactive when treated with mercaptoethanol?

Ribonuclease becomes inactive when treated with mercaptoethanol because mercaptoethanol reduces disulfide bonds in the protein, which are crucial for maintaining its three-dimensional structure. These disulfide bonds stabilize the enzyme's active conformation; when they are disrupted, the enzyme unfolds or misfolds, leading to a loss of its catalytic activity. Without the proper structure, ribonuclease cannot effectively bind to its RNA substrates.


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RNase (ribonuclease) is an enzyme that breaks down RNA molecules by cleaving the phosphodiester bonds that link RNA nucleotides together. It is involved in various cellular processes such as RNA degradation, RNA processing, and RNA quality control.


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