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Why you do staining?

Staining is done to help determine what the sample is. It makes it visible under a microscope and it gives some information about the structure of the cell. A positive Gram stain means that the cell has a thick peptidoglycan layer.


How can a mycoplasma be recognized?

Mycoplasma organisms do not contain a cell wall. Thus, traditional cell staining techniques (which require the presence of a cell wall), do not highlight the organism. A special staining technique is required for visualization.


When was the first staining done?

The Gram staining method, named after the Danish bacteriologist who originally devised it, started in 1882.


Did you stain the bacterial cell wall or the cell cytoplasm?

Yes, it is. Using the Gram staining method, the wall can be seen.


Is gram staining related to flagella?

No, gram staining and flagella are not directly related. Gram staining is a technique used to classify bacteria based on cell wall characteristics, while flagella are thread-like appendages that help bacteria move. Flagella presence or absence does not affect the results of a gram stain.


How to choose using an MTT assay vs Crystal Violet Staining?

MTT assay is used to measure cell viability by assessing metabolic activity, while crystal violet staining is used to quantify cell proliferation by staining total cellular biomass in a population. Choose MTT assay when you want to measure cell viability specifically, and crystal violet staining when you want to evaluate overall cell proliferation in a population. Each method provides different information about cell health and growth.


Why is gram staining important?

It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.


What effect does staining have on plant cells?

Staining is used to enhance the visibility of plant cell structures under a microscope by adding color to certain components such as nucleus, cell wall, or cytoplasm. It helps in studying cell morphology, identifying different cell types, and locating specific organelles within the cells. Staining can also reveal details about cell health, division, and function.


What test is done to diagnose a scratched cornea of the eye?

Fluorescien staining


Which technique enabled scientist in the S's to identify cell organelle?

Staining enabled scientist to identify cell organelles.


What is procedure is done to corneal abrasions to appear bright green?

Fluorescein staining


What is commonly found in a cell membrane?

Peptidoglycan, being the most important element for gram staining, cell differentiation....etc...