The RNA molecules that attach the amino acid to the protein chain are usually soluble.
Soluble RNA was originally called this because a soluble segment of RNA from liver cells that did not sediment (settle at the bottom/come out of suspension) after several hours of centrifuging lead to the discovery of this type of RNA. Soluble RNA is an outdated term - the current term used for this type of RNA is transfer RNA, or tRNA.
RNA is generally considered hydrophilic due to its polar nature, primarily because of its phosphate backbone and the presence of hydroxyl (-OH) groups in its ribose sugar. These features allow RNA to interact favorably with water, making it soluble in aqueous environments. However, certain regions of RNA can adopt hydrophobic characteristics when forming secondary and tertiary structures, but overall, RNA is not classified as hydrophobic.
The three types of RNA include; messenger RNA (mRNA), RNA polymerase, and transfer RNA (tRNA).
Messenger RNA (mRNA) Ribosomal RNA ( rRNA ) Transfer RNA ( tRNA )
RNA polymerase is the enzyme responsible for synthesizing RNA strands during transcription in a cell. It reads the DNA template strand and adds complementary RNA nucleotides to form an RNA strand.
Soluble RNA was originally called this because a soluble segment of RNA from liver cells that did not sediment (settle at the bottom/come out of suspension) after several hours of centrifuging lead to the discovery of this type of RNA. Soluble RNA is an outdated term - the current term used for this type of RNA is transfer RNA, or tRNA.
Ribosomes are the cellular organelles responsible for the synthesis of soluble proteins. They are composed of protein and RNA molecules. Ribosomes can be found either freely in the cytoplasm or attached to the endoplasmic reticulum.
RNA polymerase is an enzyme that makes a RNA copy from an RNA template.
RNA is generally considered hydrophilic due to its polar nature, primarily because of its phosphate backbone and the presence of hydroxyl (-OH) groups in its ribose sugar. These features allow RNA to interact favorably with water, making it soluble in aqueous environments. However, certain regions of RNA can adopt hydrophobic characteristics when forming secondary and tertiary structures, but overall, RNA is not classified as hydrophobic.
The three types of RNA are: mRNA (messanger RNA), rRNA (ribosomal RNA), and tRNA (transfer RNA).
mRNA - messenger RNA tRNA - transfer RNA rRNA - ribosomal RNA
Messenger RNA Transcription RNA Ribosomal RNA
Water soluble molecules such as protein and RNA.
The three types of RNA include; messenger RNA (mRNA), RNA polymerase, and transfer RNA (tRNA).
Messenger RNA (mRNA) Ribosomal RNA ( rRNA ) Transfer RNA ( tRNA )
RNA polymerase is the enzyme responsible for synthesizing RNA strands during transcription in a cell. It reads the DNA template strand and adds complementary RNA nucleotides to form an RNA strand.
This wash step allows you to centrifuge the sample and collect a "clean" RNA pellet, after discarding the supernatant that contained contaminating salts and proteins. When isolating and purifying RNA, 75% ethanol is used as a wash solution because RNA is a precipitate (solid) in this percentage of ethanol, while most proteins and salts remain in solution (are soluble). At a lower % ethanol, both the RNA and the proteins would be soluble, so you would not be able to separate them. At a higher % ethanol, both the RNA and salts would remain in the pellet, so you would not be able to separate the salts from your RNA. Prior to the wash step, you probably added 100% ethanol to your sample, so the final total concentration of ethanol was 75%. This step is where the RNA precipitates out of solution. You would then centrifuge the sample and discard the supernatant, as above. In the wash step, you are merely using the same solution (75% ethanol) to wash the RNA pellet you created in the previous step.