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Positive regulation of gene expression in prokaryotes occurs when a regulatory protein, often called an activator, enhances the transcription of a gene. This typically involves the binding of the activator to a specific site on the DNA, which assists RNA polymerase in initiating transcription. A classic example is the lac operon in E. coli, where the presence of lactose leads to the activation of the lac genes by the catabolite activator protein (CAP) in conjunction with cyclic AMP (cAMP).

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